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特异茶树种质紫阳1号cDNA文库构建及ESTs初步分析 被引量:5

Construction of cDNA library of Camellia sinensis cv. Ziyang 1 and primary analysis of expressed sequence tags (ESTs)
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摘要 紫阳1号是一常年不开花不结果的特异茶树种质。以紫阳1号新梢为材料,采用Creator SMARTTM cDNA Library Construction技术构建了cDNA文库。经鉴定,文库的库容量为1.0×10^6,重组率为93%,插入片段在0.5~2.0kb之间。经随机测序,获得594个有效ESTs,聚类拼接后得到299条单基因簇,包括98个重叠群和201个单拷贝。与NCBI核酸数据库进行比对分析结果表明,227个为已知功能基因或具推测功能的基因;46个为未知功能基因;26个为新的表达基因。这些数据为今后茶树功能基因分离克隆和功能基因组学研究奠定了基础。 Camellia sinensis cv. Ziyang 1 is a specific tea germplasm without flower and fruit during the whole year. A tender shoots cDNA library from the Camellia sinensis cv. Ziyang 1 was constructed by using Creator SMARTTM cDNA Library Construction Protocol. The library capacity was 1.0 ×10^6 with a recombinant rate of 93% and the inserted cDNA fragments ranged from 0. 5 to 2. 0 kb. 594 available ESTs through random sequencing were obtained, and 299 non-repetitive sequences including 98 contigs and 201 singletons were generated after clustering. 227 ESTs with known or putative function and 46 ESTs with unknown protein were identified by BlastX match of NCBI databases. The rest 26 ESTs were novel gene sequences. This study lay a foundation for gene cloning and functional genomic research of tea plant.
出处 《安徽农业大学学报》 CAS CSCD 北大核心 2009年第3期347-350,共4页 Journal of Anhui Agricultural University
基金 陕西省自然科学基金(2005C29) 西北农林科技大学博士专项基金共同资助
关键词 茶树 CDNA文库 表达序列标签 序列分析 tea plant (Camellia sinensis) cDNA library expressed sequence tag (EST) sequence analysis
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参考文献15

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