摘要
目的观察β-连环蛋白(β-catenin)和腺瘤性结肠息肉病(APC)蛋白在胎鼠下颌第一磨牙牙胚中的表达,探讨两者的作用及相互关系。方法将昆明小鼠按雌雄比2∶1合笼,发现阴栓视为妊娠,定为E0.5d,制作E13.5d、E14.5d、E15.5d、E16.5d、E17.5d胎鼠下颌第一磨牙石蜡切片,免疫组化SP法检测β-catenin和APC蛋白的表达与分布。结果β-catenin在牙胚蕾状期、帽状期、钟状期上皮内均有表达,且随着发育的成熟,其表达逐渐增强,着色于细胞膜、质及核。APC蛋白在蕾状期牙蕾上皮呈强阳性表达,随着牙胚发育其表达逐渐减弱,着色在细胞膜、质。β-catenin与APC蛋白有显著的负相关性(P<0.01)。结论β-catenin表达于牙胚早期细胞核及细胞质内,提示其参与了牙胚早期的细胞增殖过程。β-catenin与APC蛋白的时空表达有重叠现象,并呈显著负相关,这符合WNT经典信号通路中两者的作用关系。
Objective To examine the distributions of β-catenin and adenomatous polyposis coli （APC） protein in the tooth germ, and obtain the messages of function of the two factors and the relationship between them. Methods Mice were selected and cohabited with the ratio of female mice to male ones being 2：1, and Embryo day 0.5 was confirmed based on the finding of vaginal plug. The distributions of β-catenin and APC protein in the Embryos on day 13.5, 14.5, 15.5, 16.5, 17.5 were examined in the paraffin-embedded sections by immunohistochemistry methods. Results During E13.5 d to E17.5 d, positive expression of β-catenin was found in the oral epithelium and the dental lamina, and became more and more strong. The staining were whole cell. During the bud stage, strong positive expression of APC protein was found in the oral epithelium and the dental lamina, but the expression displayed a down-regulation tendency. The staining was the cytomembrane and cytoplasm. There was negative correlation between β-catenin and APC protein（P〈0.01）. Conclusion The result of β-catenin suggests its contribution in the early development of enamel organ and the proliferation of cell. Coincidance of the two factors staining site was found, according to the statistics.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2009年第4期370-373,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30700955/c03031102)
