摘要
目的：通过观察癫痫模型大鼠脑内单胺类递质水平的变化，探讨其在癫痫发病机制中的作用。方法：腹腔注射戊四氮（PTZ）建立Wistar大鼠癫痫模型，应用荧光分光光度法测定大鼠额叶、海马中多巴胺（DA）、5-羟色胺（5-HT）和去甲肾上腺素（NE）的含量。结果：癫痫模型组额叶中5-HT、DA均高于正常对照组（P〈0.05或P〈0.01）；NE含量低于对照组，两者之间的差异有显著性（P〈0.01）；海马中：癫痫模型组DA与正常对照组比较无明显差异，5-HT、NE的含量均低于正常对照组（P〈0.05或P〈0.01）。结论：脑内单胺类递质参与了癫痫活动调控，单胺水平的改变将导致脑的兴奋性改变。作用的性质取决于所在脑区，具体机制有待深入的研究。
Objective ：To study the concentrations of Monoamine in epileptic rats kindled by Pentylenetetrazol and the involvement of neurotransmitter systems as epileptogenesis modulators. Methods：PTZ （35mg/kg） intraperitoneal injection every day to establish chronic PTZ - kindling model. The concentrations of Monoamine （ dopamine （ DA ） , serotonin （ 5 - HT ） and norepinephrine （NE） ） of frontal cortex and hippocampus were determined by flurospectrophotometry. Results ： For the hippocampus determinations, 5 - HT ,NE concentrations of experimental model group were significantly lower than control group （ P 〈 0.05 or P 〈 0.01 ） ; There were no significantly dereference of the concentrations of DA between two groups （ P 〉 0.05 ）. whereas, For the frontal cortex determinations , 5 - HT,DA concentrations of model group elevated significantly as compared with control group （P 〈 0.05 ,P 〈 0.01 ）. Compared with control group, the concentrations of NE in experimental model group decreased （P 〈 0.05 ）. Conclusions： These results indicate that monoamine takes part in the epileptic process; And its effect on seizure depend on the brain area studied. Different mechanisms involved need to be further studied.
出处
《中国民康医学》
2009年第15期1774-1776,共3页
Medical Journal of Chinese People’s Health
关键词
癫痫
戊四氮
点燃
多巴胺
5-羟色胺
去甲肾上腺素
Pentylenetetrazol
5 - hydroxytryptamine
Dopamine （DA）
Serotonin （5 - HT）
Norepinephrine （NE）
