摘要
线粒体膜透化现象(MMP)被认为是大多数细胞凋亡过程的早期改变[1]。在此过程中,线粒体内膜及外膜发生不同程度的改变。外膜通透性改变引起CytC、AIF等膜间蛋白的释放,引发凋亡过程;内膜通透性改变导致线粒体跨膜电位(ΔΨm)降低,基质渗透性膨胀,进而引起外膜破裂,膜间蛋白释放从而引发凋亡。因此,对上述变化的检测,有助于凋亡的早期识别。检测MMP的实验室技术包括荧光显微镜、细胞荧光测定等,本文将对当前常用的几种方法进行分析比较。
Mitochondrial membrane permeabilization（MMP）, considered as the early stage of most apoptotic process, affects the inner and outer mitochondrial membranes（IM and OM, respectively） to a variable degree. OM permeabilization culminates in the release of proteins that normally are confined in the mitochondrial intermembrane space （IMS）, including cytochrome C （Cyt C）, apoptosis-inducing factor （AIF）,which triggers the process of apoptosis. IM permeabilization dissipates the mitochondrial transmembrane potential（ △ψ m） and promotes the osmotic swelling of the mitochondrial matrix, which results in the release of apoptogenic IMS proteins. Thus, the identification of early stage of apoptosis allows for the assessment of mitochondrial alterations as above mentioned. Several techniques to measure MMP such as cytofluorometry and fluorescence microscopy have been developed. Here, we summarize the currently available methods for the detection of MMP, and provide a comparative analysis of these techniques .
出处
《中华脑血管病杂志（电子版）》
2009年第1期33-37,共5页
Chinese Journal of Cerebrovascular Diseases(Electronic Edition)
关键词
线粒体膜透化
凋亡
Mitochondrial membrane permeabilization
Apoptosi
