摘要
目的了解高原地区大鼠严重烧伤后延迟复苏氧化应激反应对小肠上皮细胞凋亡率及凋亡相关基因bax和bcl-2表达的影响。方法将120只大鼠背部制成30％TBSAHI度烫伤后，根据海拔进行分组。高原实验组（海拔3840m）：高原即时复苏组，伤后立即腹腔注射等渗盐水40mL／kg；高原延迟复苏组，伤后6h腹腔注射等渗盐水40mL／kg。兰州实验组（海拔1517m）：兰州即时复苏组，伤后立即腹腔注射等渗盐水40mL／kg；兰州延迟复苏组，伤后6h腹腔注射等渗盐水40mL／kg。另取12只大鼠分为高原假伤组、兰州假伤组，每组6只，以37℃水浴模拟烫伤15S，伤后不补液。分别于伤后6、12、24、48、72h处死大鼠，取小肠标本检测肠组织丙二醛（MDA）和总巯基（TSH）含量；采用原位缺口末端标记法检测肠上皮细胞凋亡情况，免疫组织化学法观察bax、bcl-2基因的表达。同时留取2个假伤组小肠标本检测MDA、TSH。结果两延迟复苏组大鼠伤后肠组织中MDA含量均高于两即时复苏组（P〈0．05或P〈0．01）；伤后24h兰州延迟复苏组MDA含量为（9．8±4．0）nmol／mg、高原延迟复苏组为（10．2±1．3）nmol／mg，均分别高于兰州即时复苏组（9．54±2．7）nmol／mg和高原即时复苏组[（9．6±1．1）nmol／mg，P〈0．05]。高原实验组肠组织中TSH含量均显著低于兰州实验组；高原延迟复苏组低于兰州即时复苏组（P〈0．05）。高原延迟复苏组伤后6、12h可见大量阳性凋亡细胞，高原即时复苏组、高原延迟复苏组、兰州延迟复苏组各时相点吸光度值均高于兰州即时复苏组（P〈0．05或P〈0．01）。高原延迟复苏组伤后6、12h，肠黏膜可见大量bax阳性细胞。高原实验组大鼠bcl-2基因表达均为阴性，兰州即时复苏组和兰州延迟复苏组伤后6、12h表达均为阴性，其他时相点为弱阳性表达。结论高原地区大鼠烧伤延迟复苏后�
Objective To explore influence of oxidative stress reaction on apoptosis rate and expression of apoptosis-related genes bax and bcl-2 of enterocytes in severely burned rats with delayed resuscitation on the plateau. Methods One hundred and twenty rats subjected to 30% TBSA full-thickness scald on the back were devided into plateau experimental group （PE, altitude 3840 m） and Lanzhou experimental group （LE, altitude 1517 m）. Then LE and PE groups were subdivided into Lanzhou immediate fluid resus- citation group （ LIFR, with immediate intraperitoneal injection of isotonic saline after scald, 40 mL/kg） , Lanzhou delayed fluid resuscitation group [ LDFR, with intraperitoneal injection of isotonic saline at 6 post scald hour （PSH） , 40 mL/kg] , and plateau immediate fluid resuscitation group （PIFR, with immediate intraperitoneal injection of isotonic saline after scald, 40 mL/kg） , plateau delayed fluid resuscitation group （PDFR, with intraperitoneal injection of isotonic saline at 6 PSH, 40 mL/kg）. Another 12 rats were divided into Lanzhou sham scald group （LS） and plateau sham scald group （PS） , with 6 rats in each group. Rats in LS and PS groups were sham scalded in a water bath for 15 s without fluid infusion. Rats were sacrificed at 6, 12, 24, 48, 72 PSH for collection of small intestine samples to determine the contents of malonaldebyde （MDA） and total hydrosulfide （TSH）. The apoptosis of enterocytes was determined by TUNEL, and the expression of bax and bcl-2 in epithelial cells were observed by immunohistochemical method. Intestinal sample of LS and PS groups were collected to determine the contents of MDA and TSH. Results After being scalded, content of MDA in intestinal tissue of rats in LDFR group and PDFR group was respectively greater than that in LIFR group and PIFR group （ P 〈 0.05 or P 〈 0.01 ）. Intestinal tissue content of MDA of rats in LDFR group （9.8 ±4.0 nmol/mg） and PDFR group （ 10.2 ± 1.3 nmol/mg） was respectively greater than that inLIF
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2009年第4期289-293,共5页
Chinese Journal of Burns
基金
全军医学科学技术研究“十一五”计划专项课题（06G030）
