摘要
为降低昆虫病原线虫共生菌5-5B液体培养基的造价,使之适合规模化生产,应用二次正交旋转组合设计方法,对5-5B菌株液体培养基的组分配比进行了重新配比和优化。结果表明,在培养基各组分中,玉米淀粉对产菌量影响最大;优化后的培养基5个组分的最佳水平编码分别为0,1,2,1,-2,即玉米淀粉3.0 g·(100mL)-1,黄豆饼粉2.0 g·(100mL)-1,酵母粉2.5 g·(100mL)-1,鱼粉2.0 g·(100mL)-1,蛋白胨0.1 g·(100mL)-1。此培养基的优化配方可应用于大量生产。
The liquid medium ingredients of entomopathogenic nematode symbiotic bacteria strain 5-5B was studied for cheaper and compatible for large-scale production by the quadratic rotation-orthogonal regressive combination design. The results showed that the production of bacteria was affected mostly by corn starch in all component of the medium. The best horizontal code value of five ingredients were 0,1,2,1,-2;which meant that the five ingredients were corn starch,soybean powder,yeast,fish meal and peptone, content for 3.0,2.0,2.5,2.0 and 0.1 g.（100mL）^-1 corresponding. Over all the optimal formula of the liquid medium could be used to large-scale production.
出处
《湖北农业科学》
北大核心
2009年第7期1633-1636,共4页
Hubei Agricultural Sciences
基金
农业部"948"项目(2001238)
沈阳农业大学青年教师科研基金资助项目(2005002)
关键词
昆虫病原线虫共生菌
液体培养基
二次正交旋转组合设计
entomopathogenic nematode symbiotic bacteria
liquid medium
the quadratic rotation-orthogonal regressive combination design
