摘要
通过研究壬基酚对黑斑蛙(Rana nigromaculata)血浆渗透压以及血细胞的影响,探讨壬基酚对黑斑蛙血液的毒性效应。用200、400和600mg/kg壬基酚分别对黑斑蛙腹部淋巴囊注射染毒,在不同的时间间隔内利用渗透压仪测量各组血浆渗透压,同时制作血涂片观察血细胞的异常现象。结果表明,在相同处理时间内,随着壬基酚浓度的增加,黑斑蛙血浆渗透压值上升,血细胞膨大,血细胞核分裂以及核质不均匀现象明显;在相同浓度处理组中,随着处理时间的延长,黑斑蛙血浆渗透压上升,血细胞膨大,细胞核损害严重。壬基酚可诱发红细胞出现微核现象,随着壬基酚浓度的增加,同一处理时间内黑斑蛙红细胞微核及核异常率呈现先上升后下降的变化规律;随着处理时间的延长,各处理组红细胞微核率及核异常率呈现下降的趋势。
To study the toxic effect of nonylphenol on Rana nigromaculata blood, osmotic pressure and blood cells were researched. 200, 400 and 600 mg/kg nonylphenol were injected in R. nigromaculata by the abdominal lymph capsule separately, and a vapor pressure osmometer was used to measure blood osmotic pressure in different time. At the same time, blood smears were done to observe the abnormalities of blood ceils. The results showed that at the same treated time, plasma osmolality increased in R. nigromaculata with the increasing concentration of nonylphenol. The blood cells enlarged, blood cell nucleolus splited, the phenomenon of uneven quality were observed obviously. At the same treated group, with the increasing concentration of nonylphenol, blood osmotic pressure increased, blood cells enlarged, the nucleus damage was aggravated. Nonylphenol could induce the micronueleus （MN） and other nuclear anomalies （ONA） in erythrocytes of peripheral blood. With nonylphenol concentration increasing, the rate of MN and ONA increased first and decreased last. The rate of MN and ONA decreased with the treated time expand.
出处
《动物学杂志》
CAS
CSCD
北大核心
2009年第4期129-134,共6页
Chinese Journal of Zoology
基金
安徽省教育厅自然科学基金项目(No.2006kj-222B)
安徽省高校青年教师科研项目(No.2006jql214)
关键词
壬基酚
黑斑蛙
血液
毒性效应
微核
Nonylphenol
Rana nigromaculata
Blood
Toxic effect
Micronucleus
