摘要
目的探讨宫颈癌患者新辅助化疗与放疗前后外周血T细胞亚群变化。方法采用流式细胞仪技术,检测外周血中T细胞亚群、NK细胞含量,并与对照组比较。结果宫颈癌治疗前CD3+、CD4+、CD8+、CD4+/CD8+的含量均较对照组低,无显著性差异(P>0.05);NK值较对照组高,有显著性差异(P<0.05);不同分期间比较无显著性差异(P>0.05)。化疗后CD3+、CD4+、CD8+、CD4+/CD8+的含量和NK值均逐渐降低,与治疗前比较有显著性差异(P<0.05);放疗后CD3+含量、NK值继续下降,CD4+、CD8+含量和CD4+/CD8+比值略有升高,与治疗前比较有显著性差异(P<0.05);而化疗后和放疗后外周血中T细胞亚群、NK细胞含量比较均无显著性差异(P>0.05)。结论新辅助化疗与放射治疗会降低宿主机体免疫功能,提示化疗和放疗期间应及时辅以免疫治疗,以增强机体的免疫功能。
Objective To explore the peripheral blood T cell subsets change of cervical cancer patients with pre-and post adjuvant chemotherapy and radiotherapy. Methods Using flow cytometry to detect peripheral blood T cell subsets, NK cells compared with the contrast group. Results The CD3 ＋ ,CD4 ＋ ,CD8 ＋ ,CD4 ＋/CD8 ＋ in the pretreatment group is lower than the contrast group,There was no significant difference （P 〉 0.05 ）between them; NK value was higher than the contrast group, there was a significant difference（ P 〈 0.05 ） ; There was no significant difference in different stage groups（ P 〉 0.05 ） ; The CD3 ＋ ,CD4 ＋ ,CD8 ＋ ,CD4 ＋/CD8 ＋ and NK value had been gradually reduced after chemotherapy compared with the pre-treatment, There were significantly difference （P 〈 0.05 ） ;The CD3 ＋ , NK continued to decline after Radiotherapy, while CD4 ＋ , CD8 ＋ and CD4 ＋/CD8 ＋ increased slightly compared with the pre-treatment, there were significantly difference （ P 〈 0.05 ）. And after chemotherapy and radiotherapy, the change of peripheral blood T cell subsets and NK value show no significant difference （P 〉 0.05 ）. Conclusion The adjuvant chemotherapy and radiation therapy will decrease the host immune function. We suggest immunotherapy should be supplemented during chemotherapy and radiotherapy, so as to enhance immune function.
出处
《实用癌症杂志》
2009年第3期248-250,共3页
The Practical Journal of Cancer
关键词
宫颈癌
新辅助化疗
放疗
T淋巴细胞亚群
NK细胞
流式细胞术
Cervical cancer
Neoadjuvant chemotherapy
Radiotherapy
T-lymphocyte subsets
NK cells
Flow cytometry
