摘要
从竹红菌中提取竹红菌甲素，将其转化为竹红菌乙素，建立了HPLC测定合成竹红菌乙素含量的方法。采用Shim-pack VP-ODS色谱柱，色谱条件：柱温为30℃；甲醇-水（体积比83：17）为流动相；紫外检测波长300nm。竹红菌乙素的保留时间为11.5min。竹红菌乙素质量在0.29-1.45μg时，峰面积与质量的线性关系良好（r=0.9993），回收率为98.3％，RSD为1.2％（n=5）。该分析方法简便、快速，对于竹红菌乙素合成过程中转化率测定提供一定的理论依据。
Hypocrellin A was extracted from Hypocrella Bambusesae, then synthesized into Hypocrellin B. HPLC was used for the determination of Hypocrellin B . The suitable chromatogram conditions were as follows： Shim-pack VP-ODS column was as the stationary phase at 30℃, the mobile phase was methanol-water （83：17 by volume）, and the detection wavelength was 300 nm. The save time of Hypocrellin B was 11.5min. There was a good linear relationship （r=0.9993） between mass concentration and peak area in the range of 0.29-1.45μg. The average recovery was 98.3%, and RSD was 1.2% （n=5）. The method is simple and quick, therefore can be used for the determination of Hypoerellin B in synthesis.
出处
《黑龙江八一农垦大学学报》
2009年第3期70-72,共3页
journal of heilongjiang bayi agricultural university
基金
黑龙江八一农垦大学引进硕士科研启动基金(校启S2004-054)
