摘要
目的:建立同时测定竹花药材中竹红菌甲素、竹红菌乙素、竹红菌丙素含量的方法。方法:采用HPLC,KromasilC18色谱柱(4.6 mm×250 mm,5μm),柱温35℃,以0.5%冰醋酸水溶液和乙腈为流动相,检测波长265 nm,流速1.0 mL.min-1。结果:3种成分均达到基线分离,线性关系良好,精密度RSD<2.0%。该法成功的用于竹花药材中3种成分含量的测定,药材中竹红菌甲素、乙素、丙素的平均回收率分别为101.8%,102.3%,100.0%。结论:该方法操作简单、结果准确,具有较好的重复性和稳定性,为竹花药材质量控制提供了一个很好的依据。
Objective: To develop a high-performance liquid chromatography(HPLC) method for simultaneous determination of hypocrellin A,hypocrellin B,and hypocrellin C.Method: The separation was carried out on a Kromasil C18(4.6 mm×250 mm,5 μm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile.The column temperature was 35 ℃,and the flow rate was 1.0 mL·min-1.The detection wavelength was set at 265 nm.Result: The three compounds were well separated.Calibration curves of hypocrellin A,hypocrellin B,and hypocrellin C showed good linear relationship RSD>2.0%.The average recoveries of the hypocrellin A,hypocrellin B,and hypocrellin C were 101.8%,102.3%,100.0%,respectively.Conclusion: The developed method is simple,accurate,and repeatable,and can be readily used as valid tool for the quality control of Hypocrella bambusae.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第1期75-78,共4页
China Journal of Chinese Materia Medica
基金
江苏省国际合作项目(BZ2009097)
