摘要
经过初筛和复筛从土样中分离出1株高产纤维素酶真菌SNB9,经形态学和ITS序列分析,鉴定为黑曲霉(Aspergullus niger)。生长条件的测定显示该菌生长范围偏酸。发酵后纤维素酶的最适作用pH在4.0～5.0,最适作用温度在45～55℃。滤纸酶活为9.29U/mL,C1酶活为23.69U/mL,CMCase酶活为38.23U/mL,β-葡萄糖苷酶活为65.52U/mL。发酵液中除了纤维素酶,还发现有辅助酶,包括木聚糖酶、淀粉酶、果胶酶、蛋白酶。
A cellulase high-producing fungus strain SNB9 was isolated from soil samples after proto-screening and re- screening and characterized as Aspergillus niger after morphological and ITS sequencing analyses. Growth conditions tests indicated that it grew on recta-acidic range. And the most suitable reactive pH of the eellulase was 4.0 - 5.0, and temperature 45 -55℃. The activities of FPA, C1, CMCase, and 13-glycosidase were 9.29 U/mL, 23.69 U/ mL, 38.23 U/mL, 65.52 U/mL respectively. Besides cellulase, it was found there were some other auxiliary en- zymes in the fermented broth, including xylanase, amylase, pectinase, and protease.
出处
《微生物学杂志》
CAS
CSCD
2009年第3期72-76,共5页
Journal of Microbiology
基金
辽宁省自然科学基金(20062109)
沈阳市科技计划项目(1063312-1-00)
关键词
筛选
纤维素酶
鉴定
黑曲霉
screening
cellulase
characterization
Aspergillus niger
