摘要
目的：研究滇重楼根部丛枝菌根真菌（AMF）侵染情况及根际土中AMF孢子的种类与数量。方法：采用Phillips和Hayman染色法挑选滇重楼丛枝菌根。同时,采用Gendemann的湿筛倾析法获得AMF孢子,进行形态学鉴定。结果：丛枝菌根真菌能侵染滇重楼根系并形成典型的丛枝菌根（AM）,侵染率在35.3%~98.6%,且侵染强度较强。从滇重楼根际采集的10个土样中共分离出24种AMF,Acaulospora属11种,Glomus属7种,Gigaspora属3种,Scutellospora属3种,分别是附柄无梗囊霉、双网无梗囊霉、凹坑无梗囊霉、孔窝无梗囊霉、浅窝无梗囊霉、光壁无梗囊霉、柯氏无梗囊霉、多果无梗囊霉、波兰无梗囊霉、瑞氏无梗囊霉、细凹无梗囊霉、白色球囊霉、双型球囊霉、沙荒球囊霉、草莓球囊霉、纯黄球囊霉、微丛球囊霉、凹坑球囊霉、微白巨孢囊霉、珠状巨孢囊霉、分支巨孢囊霉、美丽盾巨孢囊霉、透明盾巨孢囊霉、吉尔莫盾巨孢囊霉。其中,双网无梗囊霉是滇重楼的优势种。结论：AMF是滇重楼丰产栽培中的一种潜在应用价值的生物资源。
Objective： To study the infection situation of arbuscular mycorrhizal fungi, as well as the mycorrhizal structures of Paris polyphylla var. yunnanensis, and the main types and quantities of AMF spores in rhizosphere soil. Method： The arbuscular my- corrhizal of P. polyphyUa var. yunnanensis were detected by Phillips and I-Iayman staining. At the same time, some AMF spores were accessed by Gendemanng Wet-screening method and identified by their morphological characteristics. Result： Arbuscular mycorrhizal fungi could infect the roots of P. polyphylla var. yunnanensis and formed arbuscular mycorrhizal. Infection rate was from 35.3 % to 98.6 %, indicating that infection strength was strong. From 10 soil samples collected in Yunnan, I 1 Acaulospor species, 7 Glomus species, 3 Gigaspora species and 3 Scutellospora species were isolated and identified, including Acaulospora appendicola, A. brieticula- ta, A. excavata, A. foveata, A. lacunosa, A. laevis, A. koskei, A. myriocarpa, A. polonica, A. rehmii, A. scrobiculata, Glomus al- bidum, G. ambisporum, G. deserticola, G. fragarioides, G. luteum, G. microaggregatum, G. multiforum, Gigaspora albida, G. mar- garita, G. ramisporophora , ScuteUospora calospora , S. pellucida and S. gilmorei. Among them, Acaulospora brieticulata was advan- tage species. Conclusion： AMF may be a potent biological resource which can stimulate the growth of P. polyphylla var. yunnanensis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第14期1768-1772,共5页
China Journal of Chinese Materia Medica
基金
大理学院药物研究所科研项目(2008YY006)
关键词
滇重楼
丛枝菌根真菌
丛枝菌根
双网无梗囊霉
Paris polyphylla var. yunnanensis
arbuscular mycorrhizal fungi
arbuscular mycorrhizal
Acaulospora brieticulata
