摘要
目的探讨激素性股骨头坏死的发病机制。方法24只新西兰大白兔随机分为实验组(冲击注射地塞米松)和对照组。实验8周处死动物,取股骨头作骨细胞组织病理学、超微结构观察,并用TUNEL及免疫组化技术分别检测骨细胞凋亡和Bcl-2表达情况。结果光镜下实验组较对照组股骨头骨小梁变细、稀疏、空骨陷窝明显增多,脂肪细胞增大。电镜下实验组骨细胞体积缩小,核固缩,线粒体及高尔基体肿胀,染色质边聚较多见。实验组凋亡骨细胞增多,Bcl-2表达骨细胞轻度增加。结论激素性股骨头坏死是骨细胞凋亡和坏死共同作用的结果。
Objective To observe necrosis, apoptosis and the expression of Bcl - 2 protein of osteocytes in dexamethasone（DEX） - induced osteonecrosis of the femoral head（ONFH） in rabbits and study the pathogenesis of the DEX - induced ONFH. Methods Twenty - four adult rabbits were randomly divided into experimental group（ injected with massive dose of DEX） and control group. Pathological changes, apeptosis and the expression of Bcl -2 protein of the femoral heads were observed in 8 weeks. Results The trabecula of femoral head of the experimental group changed thinner and sparser, empty bone lacune increased, and the bone marrow cavity with edema and larger fatty cells were also observed and compared with them in the control group. Under electron microscope, experimental group showed a great deal of necrotic osteocytes with pyknotic nucleus, the cytoplasm and organell were swelling. Apoptosis and the expression of Bcl - 2 protein in experimental group were more than that of control group. Conclusions Necrosis and apoptosis of osteocytes are coagents of the steroid - induced osteonecrosis of the femoral head.
出处
《医学信息（内.外科版）》
2009年第6期516-517,共2页
Medical Information Operations Sciences Fascicule
