摘要
目的观察肝癌HepG2细胞转移相关基因CD44v6、EzfinmRNA表达及三氧化二砷（As2O3）对其表达的影响。方法以逆转录-聚合酶链反应（RT—PCR）方法检测HepG2细胞中CIM4v6、Ezfin基因mRNA的表达及As203作用后其表达水平的变化。结果HepG2细胞CD44v6及EzfinmRNA均有表达。CIM4v6-mRNA表达水平为1．57±0．47；经0．25mg／L As2O3作用4、6、8d后其表达水平分别下降到0．95±0．25、0．90±0．24和0．63±0．19（P〈0．05），下降水平呈时间依赖；Ezfin．mRNA表达水平为0．70±0．18，加入0．25mg／L的As20，4、6、8d后，表达水平分别为0．70±0．21、0．75±0．22和0．71±0．20，各时间段Ezfin—mRNA的表达水平无明显变化（P〉0．05）。结论As2O3可显著下调HepG2细胞CD44v6-mRNA的表达，对Ezfin-mRNA的表达可能无直接调节作用。
Objective To investigate the expression of CD44v6,and Ezrin mRNA in human hepatocellular carcinoma cells （HepG2） ,and evaluate the effects of arsenic trioxide （As2O3 ） on the expression in HepG2 in vitro. Methods The expression levels of CD44v6 and Ezrin mRNA were examined by RT-PCR in HepG2 cells in vitro. Results The expression of CD44v6 and Ezrin mRNA was detected in HepG2 cells. The expression levels of CD44v6 and Ezrin mRNA were 1.57±0.47 and 0.70 ±0.18,respectively. A significant down-regulation of expression of CD44v6 mRNA was observed in HepG2 cells when 0.25 mg/L As2 O3 was given at the 4th,6th, and 8th day （0.95 ± 0.25,0.90 ± 0.24, and 0.63 ± 0.19 ,P 〈 0.05）. No change was found in the expression of Ezrin mRNA when HepG2 ceils were treated with As2O3 for 4,6, or 8 days （0.70 ± 0.21,0.75 ± 0.22, and 0.71 ± 0.20, P 〉 0.05 ）. Conclusion As2O3 can decrease the expression of CD44v6 mRNA, but it can not directly regulate the expression of Ezrin mRNA in HepG2 ceils in vitro.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第8期986-987,共2页
Chinese Journal of Experimental Surgery
基金
湖北省科技厅攻关计划资助项目（2006AA301C28）
