摘要
目的:探讨TMEM16A在食管鳞状细胞癌发生发展中的意义.方法:2007-01/2008-04南京医科大学附属江宁医院手术切除的食管鳞癌标本60例,均为癌组织及距癌至少5cm远的配对癌旁组织.应用免疫组织化学SP法及Western blot法检测食管鳞癌组织及相应癌旁组织的TMEM16A蛋白表达.结果:免疫组织化学结果:食管鳞癌组织、癌旁组织中TMEM16A蛋白的表达差异具有统计学意义(52.50%vs20.00%,P<0.05),TMEM16A在淋巴结转移组中的阳性表达率显著高于无淋巴结转移组(65.38%vs21.43%,P<0.05),不同分化程度高分化、中分化、低分化的食管鳞状细胞癌中,TMEM16A的阳性表达率分别为27.27%、43.75%和84.62%,3者比较差异有显著性(P<0.05).TMEM16A表达与食管鳞癌的TNM分期无明显相关性;Western blot法检测发现TMEM16A在食管癌组织的表达较癌旁组织强(t=42.16,P<0.05).结论:TMEM16A可能在食管癌的发生中有一定的作用,有望为食管癌的分子诊断、基因治疗和预后评估提供新的分子靶点.
AIM： To identify the expression of TMEM16A in esophageal squamous cell cancer （ESCC） and to explore its potential role in carcinogenesis and progression of esophageal cancer. METHODS： A total of 60 cases with esophageal squamous cell carcinoma, which were cancerous tissues and their adjacent tissues, were collected at Jiangning hospital affiliated Nanjing medical university from 2007-01 to 2008-04. All tumor tissues were matched with distant tissues at least 5 cm away from cancer. Im- munohistochemistry staining （SP method） and Western blot were performed to detect protein TMEM16A expression in ESCC and its corre-sponding para-cancerous tissues RESULTS： Immunohistochemistry revealed that the positive rate of TMEM16A protein was 52.50% in carcinoma of esophagus, significantly higher than that in cancer-adjacent tissues （20%, P 〈 0.05）. There was significant difference in TMEM16A expression between the lymph node positive group and the lymph node negative group （65.38% vs 21.43%, P 〈 0.05）. The positive rates of TMEM16A protein in tumor of high, middle and low differentiation groups were 27.27%, 43.75% and 84.62%, respectively, with a statistically significant difference （P 〈 0.05）. No significant association was observed between the positive expression of TMEM16A and TNM stages; we also found a higher expression of TMEM16A in ESCC than in para-cancer tissues by Western blot （t = 42.16, P 〈 0.05）. CONCLUSION： TMEM16A may play an important role in carcinogenesis of ESCC. TMEM16A is expected to be a new significant biomarker in molecular diagnosis, gene therapy, and progno- sis evaluation for ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第15期1513-1516,共4页
World Chinese Journal of Digestology
