摘要
目的建立复方芩柏颗粒中黄芩苷、盐酸小檗碱含量测定的高效液相色谱法。方法采用Diamonsil C18柱,以甲醇-水(含0.05%磷酸和0.1%三乙胺)为流动相进行梯度洗脱,检测波长276nm。结果在该色谱条件下黄芩苷、盐酸小檗碱分别在10.08～201.6mg·L-1、4.08～81.6mg·L-1浓度范围内呈良好的线性关系。其平均回收率分别为98.1%、97.9%,RSD分别为1.11%、1.24%。结论该法简便可行、重复性好,可为复方芩柏颗粒制剂的质量控制标准提供参考依据。
Objective To establish a HPLC method for the determination of baiealin and berberine hydrochloride in Compound Qinbo Granules. Methods The gradient elution was performed on a Diamonsil C18 column using method - water （0. 05 % phosphate acid and 0. 1% trithylamine） as mobile phase, and the detection wavelength was 276 nm. Results The linear calibration curve of baicalin was obtained in the range of 10. 08 -201.6 mg · L^-1 with an average recovery of 98. 1% （RSD = 1.11% ）. The linear calibration curve of berberine hydrochloride was in the range of 4.08 - 81.6 mg· L^-1 with an average recovery of 97.9 % （RSD = 1.24 % ）. Conclusion The method is simple and reproducible. It can be used for the quality control of Compound Qinbo Granules.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第4期349-351,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
