摘要
将发根农杆菌ＭＡＦＦ０３－０１７２４株系，涂布于逆向插入未添加任何生长调节剂的ＭＳ培养基中的紫果西番莲的茎段切口上．４周后，切口上出现瘤状突起，次周产生不定根．切下不定根根尖，培养于含Ｃｌａｆｏｒａｎ０．５ｇＬ－１的培养基中，分化出发状根．发状根转入含有ＢＡ１ｍｇＬ－１和２，４－Ｄ０．１ｍｇＬ－１的ＭＳ培养基中，分化出植株．再生植株经滤纸电泳检测，测出了农杆碱和甘露碱，说明紫果西番莲已发生了遗传转化．
Agrobactium rhizogenes MAFF0301724 was smeared over the stem segment of Passiflora edulis inoculated upsidedown into MS solid medium. After 4 weeks, tumors broke out of the injured stem segment. The adventitious root was derived from the tumors next week. The tip of adventitious root was cut and cultured in the medium containing 0.5 g·L-1 claforan for differentiating hairy roots. The hairy roots were transferred into MS medium supplmented with BA 1 mg·L-1 and 2,4D 0.1 mg·L-1, they developed into wholly plantlets later. Man. and Agr. were detected in the samples of plantlets, it showed that the plantlets of P. edulis was transformed.
出处
《福建农业大学学报》
CSCD
1998年第2期255-256,共2页
Journal of Fujian Agricultural University
基金
国家教委资助
关键词
发根农杆菌
紫果西番莲
遗传转化
Agrbactirum rhizogenos
Passiflora edulis
genetic transformation
