摘要
以紫果西番莲离体培养的子叶为实验材料,对其愈伤组织进行诱导、分化及不定芽增殖。结果表明:在愈伤组织诱导中,以MS培养基+2,4-D 1.0 mg/L+TDZ 0.5 mg/L+琼脂3.8 g/L+蔗糖30 g/L的诱导效果最好,其诱导率为73.33%。在愈伤组织不定芽诱导中,以MS培养基+2,4-D 0.5 mg/L+TDZ 2.0 mg/L+琼脂3.8 g/L+蔗糖30 g/L的诱导率最佳,为46.67%。对不定芽继续进行增殖培养,以MS培养基+6-BA 1.0 mg/L+IAA 0.3 mg/L+琼脂4.0 g/L+蔗糖30 g/L最佳,增殖倍数为2.97。从而建立了西番莲植株再生体系,可进行优良材料的离体快繁。
Cotyledons of Passiflora caerulea were used as experimental materials to induce callus, differentiation and adventitious buds proliferation. The results showed that the callus induction medium using MS+2,4-D 1.0 mg/L+TDZ 0.5 mg/L+Agar 3.8 g/L+Sucrose 30 g/L had the highest rate of callus induction, and the induction rate was 73.33%. The optimum medium for inducing buds was MS+2,4-D 0.5 mg/L+TDZ 2.0 mg/L+Agar 3.8 g/L+ Sucrose 30 g/L, the induction rate reached 46.67%.The optimum medium for further culturing adventitious buds was MS medium+6-BA 1.0 mg/L+IAA 0.3 mg/L+Agar 4.0 g/L+Sucrose 30 g/L, the multiplication multiple was 2.97. The established plant regeneration system could provide technical support for rapid propagation in vitro, ploidy breeding and genetic transformation of P. caerulea.
作者
王亚楠
张湛仪
赵李姗
余俊玲
汪梦婷
蔡年辉
唐军荣
许玉兰
Wang Yanan;Zhang Zhanyi;Zhao Lishan;Yu Junling;Wang Mengting;Cai Nianhui;Tang Junrong;Xu Yulan(Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming Yunnan 650233, China;Key Laboratory of Ministry of Education for Southwest Mountain Forest Resource Conservation and Utilization, Southwest Forestry University, Kunming Yunnan 650233, China;Yunnan Provincial Key Laboratory of Forest Genetic Improvement and Breeding in Universities of Yunnan, Southwest Forestry University, Kunming Yunnan 650233, China;Sharihaolai Forestry Workstation, Forestry Bureau of Naiman Banner, Tongliao Inner Mongolia 028300, China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2019年第3期155-160,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
云南省高校林木遗传改良与繁育重点实验室基金(YNGBS201705)资助
关键词
西番莲
子叶
愈伤组织
分化
不定芽
增殖
Passiflora caerulea
cotyledons
callus
differentiation
adventitious buds
proliferation
