摘要
以日本石楠[Photinia glabra(Thunb.)Maxim.]为材料,建立了一套高效的适合工厂化生产的腋芽再生组织培养体系。该体系包括芽诱导培养基(MS+1.2mg.L-1BAP+0.2mg.L-1NAA),继代培养基(WPM+0.75mg.L-1BAP+0.15mg.L-1NAA)和生根培养基(1/2MS+2.5mg.L-1IAA+0.3mg.L-1IBA)。在培养出的超过10万株的组培再生苗中,随机选出36株和24株进行AFLP和MSAP检测遗传和DNA甲基化的变化。在检出的615条AFLP条带和392条MSAP条带中,并未发现任何变异,表明建立的腋芽再生培养体系稳定可靠,适合于日本石楠的工厂化生产。
We established a tissue culture system for efficient micropropagation of Japanese photinia [ Photinia glabra （ Thunb. ） Maxim. ] by enhanced branching of axillary buds taken from branches of a single donor tree. The culture system consists of choosing the suitable explant coupled with sequential use of three media, namely, the bud-induction medium （ MS medium supplemented with 1.2 mg·L^-1BAP, 0. 2 mg·L^-1NAA）, subculture medium （WPM medium added with 0.75 mg·L^-1BAP, 0. 15 mg. L-1NAA） and root-induction medium （half-strength MS medium fortified with 2.5 mg·L^-1 IAA and 0. 3 mg·L^-1 IBA）. In addition, by using AFLP and MSAP markers we investigated the genetic and DNA methylation pattern stability of samples of 36 and 24 morphologically normal plants respectively, which were randomly taken from a popu- lation of more than 100 000 micropropagated plants established in the field. We found that of the 615 and 392 reproducible bands scored respectively for AFLP and MSAP, no evidence for occurrence of genetic or epigenetic instability. This, together with phenotypic uniformity of the micropropagated population, suggests that micropropagation through enhanced axillary bud branching ensured genetic and epigenetic fidelity of the donor plant in Japanese Photinia. Therefore, the protocol reported here can be readily employed for large-scale commercial propagation of this ornamental plant species.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第7期1071-1076,共6页
Acta Horticulturae Sinica
基金
国家转基因植物研究与产业化开发专项项目(JY04-B-02)
关键词
日本石楠
腋芽再生
遗传/表观遗传稳定性
Photinia glabra （Thunb.） Maxim.
axillary bud
genetic/epigenetic stability
