摘要
目的探讨复方苦参对人γδT细胞杀伤胃癌细胞株SGC-7901的影响。方法用异戊烯焦磷酸法体外扩增人外周血γδT细胞，用不同浓度的复方苦参诱导γδT细胞SGC-7901细胞株24h，用MTT法检测复方苦参对这两种细胞生长抑制率的影响和LDH法检测γδT细胞的杀伤活性，用流式细胞术检测诱导前后的18T细胞和SGC-7901的凋亡率。结果γδT细胞培养10d时从扩增前4．21％增加到70．35％，CD44达94．0％。不同浓度的复方苦参对SGC-7901细胞株的抑制率（22．3％）均明显高于γδT细胞（-22．4％），且γδT细胞的抑制率呈负的趋势，当复方苦参的浓度在1／50～1／400时γδT细胞负抑制率呈剂量依赖关系，且经复方苦参诱导24h的γδT细胞杀伤活性（83．6％）明显高于先诱导SGC-7901组（71．2％），同时经复方苦参诱导24h的18T细胞凋亡率（4．64％）明显低于SGC-7901（49．23％）。结论复方苦参在临床常规使用浓度下，能够促进78T细胞的增殖，同时能够抑制肿瘤细胞的生长，且能够增强γδT细胞的杀伤活性，这一结果将有助于肿瘤的过继免疫治疗及为复方苦参应用于肿瘤治疗提供了临床依据。
Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT ceils and gastric cancer cell lines SGC- 7901 for 24 hours, MTT assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoPtosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ratio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkushen on SGC-7901 at various concentrations was significantly higher than that on γδT cells（22.3% vs-22.4% , P 〈 0.05 ）. The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen＇ s concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, （83.6% vs 71.2% ,P 〈 0.05）. Apoptotic rate was significantly lower in γδT cells than in SGC-7901 （4.64% vs 49.23%, P 〈 0. 05）. Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells＇ proliferation, inhibit tumor cell growth and enhance γδT cells＇ cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the application of Fufangkushen in the treatment of tumors.
出处
《国际免疫学杂志》
CAS
北大核心
2009年第4期262-266,330,共6页
International Journal of Immunology
