摘要
目的探讨加味大柴胡汤(MMDB)对重症胆管炎(ACST)大鼠TLR4表达的影响。方法大鼠分为正常组、造模组、减压组、中药组。制作ACST大鼠模型后,分别于24、48及72h3个时点检测内毒素、肝功能及用RT-PCR检测TLR4mRNA的表达。结果造模组内毒素水平和TLR4含量均比正常组高;MMDB配合胆管减压治疗后,内毒素水平下降,TLR4表达降低,总胆红素降低,肝脏损害减轻。结论MMDB可以通过下调TLR4mRNA表达来降低血浆内毒素水平,从而减轻肝脏损害。
Objective To observe the influence and mechanism of modified major decoction of bupleulrum （MMDB）on TLR4 expression in rats with acute cholangitis of severe type（ACST）. Methods After ACST models were established, the rats were randomly divided into model group, internal choledochal drainage group and medical herbs group. The changes of endotoxin and liver function were measured and the expression of TLR4 mRNA in the liver was detected using RT-PCR at three time points of 24,48 and 72 h. Results The expression of TLR4 mRNA and endotoxin levels of ACST model group were higher than those of normal control rats, which were decreased with reduced liver injury after treatment with MMDB combined with internal choledochal drainage. Conclusion MMDB could reduce the density of endotoxin in blood and alleviate the liver injury by down-regulating the expression of TLR4 mRNA.
出处
《江苏医药》
CAS
CSCD
北大核心
2009年第7期831-833,共3页
Jiangsu Medical Journal
关键词
急性胆管炎
加味大柴胡汤
TLR4
Acute cholangitis
Modified major decoction of bupleulrum
Toll-like receptor 4
