摘要
目的探讨昆明山海棠提取物(THW-4)对人脐静脉血管内皮细胞增殖的抑制作用.方法培养人脐静脉血管内皮细胞,加入不同计量的THW-4进行处理,细胞计数、MTT、细胞周期测定观察THW-4对HUVEC增殖的影响.结果THW-4可以明显抑制HUVEC增殖,呈剂量依赖性,作用24h的半有效抑制浓度(IC5)0为1.09ng/mL;THW-4(0.5～10ng/mL)作用HUVEC24h后,THW-4主要阻滞HUVEC于G0/G1期.结论THW-4能有效抑制体外培养的HUVEC增殖。
Objective To study the effect of THW-4, an extract from Trypterygium hypoglaucum on the proliferation of human umbilical vein endothelial cells （HUVEC）. Methods HUVEC were cultured in vitro and different concentrations of THW-4 were added in experimental groups. Cell proliferation was evaluated by cell counting, MTT and and cell cycle detection. Results The inhibitory effects of THW-4 on proliferation of HUVEC displayed a dose dependent manner, with the haff-inhibition concentration value （IC50） of 1.09 ng/mL at 24 h. Analysis of cell cycle indicated the THW-4 （0.5 - 10 ng/mL） mainly lead to the blockage of HUVEC in G0/G1 stage. Conclusion THW-4 has an inhibitory effect on the proliferation of HUVEC in vitro, and it has no prolection on regeneration of functional endothehum after artery balloon.
出处
《昆明医学院学报》
2009年第7期41-45,共5页
Journal of Kunming Medical College
关键词
昆明山海棠
提取物
人脐静脉血管内皮细胞
细胞增殖
Tripterygium hypoglaucum （Levl.） Hutch
Extract
Human umbilical vein endothelial cell
Cell proliferation
