摘要
[目的]阐明重金属Pb/Zn复合污染对香樟生理的影响,为利用香樟进行尾矿恢复提供技术支持。[方法]以香樟为试验材料,采用土培盆栽方法,研究不同复合重金属Pb/Zn浓度(0、200、400和800 mg/kg)对樟树叶和根中的叶绿素含量、抗氧化酶(SOD、POD和CAT)活性、丙二醛(MDA)和脯氨酸(Pro)含量的影响。[结果]随着复合重金属处理浓度的增加,叶绿素全量、叶绿素a含量和叶绿素b含量呈现明显的下降趋势,但是叶绿素a/b比值只在复合重金属浓度为800 mg/kg时明显下降。复合重金属对樟树幼苗抗氧化酶活性有着不同的影响作用,对POD呈现明显的抑制作用,对SOD则表现出促进的作用,而对CAT则是呈明显的低促高抑现象。随着重金属处理浓度的升高,叶和根中的MDA和Pro呈现明显增加的趋势。[结论]重金属胁迫对香樟的光合作用产生了抑制作用,对抗氧化酶活性表现出不同的影响作用。
[ Objective ]The study aimed to study the effects of complex heavy metals （Pb/Zn） on physiological reponses of P. fortunel, and provide theoretical guidance for re-vegetating mine tailing with P. fortunel.[ Method ] A pet experiment was performed to investigate the effects of different concentration （0,200,400 and 800 mg/kg） of complex heavy metals on chlorophyll contents, antioxidant enzyme activities （SOD, POD and CAT）, MDA and praline contents. [ Result ] Along with the increase concentration of complex heavy metals, chlorophyll a, chlorophyll b and total chlorophyll contents significantly decreased, but the chlorophyl a/b ration only significantly decreased at 800 mg/kg. Complex heavy metals had different effects on specific antioxideant enzyme. Complex heavy metals had significant restrain effects on POD, significant promoting effects on SOD, and promoting effects on CAT at low concentration but restrain effects on CAT at high concentration. Moreover, the contents of MDA and proline significantly increased along with the concentration of complex heavy metals. [ Conclusion]Complex heavy metals had restrained effects on chlorophyll contents of P. fortunel and had different effects for specific antioxidant enzymes of P. fortanel.
出处
《安徽农业科学》
CAS
北大核心
2009年第21期10253-10254,10257,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省自然科学基金(Y507053)
关键词
香樟
重金属
叶绿素
抗氧化酶
丙二醛
脯氨酸
Paulowniu fortunel （seem） Hems.
Heavy metals
Chlorophyll
Antioxidant enzyme
Malondialdehyde
Proline
