摘要
背景与目的:既往研究显示趋化因子受体CCR7在胃肠道肿瘤中表达上调,并与淋巴管浸润和淋巴结转移相关。本研究旨在探讨趋化因子CCL21及其受体CCR7在人结肠癌SW480细胞体外侵袭中的作用。方法:在趋化因子CCL21作用的条件下,用划痕实验和Transwell小室检测SW480细胞侵袭能力,免疫印迹检测SW480细胞中金属基质蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达;CCL21预处理的SW480细胞在含足叶乙甙(VP-16)的体系中培养,MTT法检测细胞增殖活力,流式细胞术和Hoechst33258染色检测细胞凋亡。结果:CCL21(100ng/mL)组中SW480细胞向划痕处爬行的速度明显快于阴性对照组;阴性对照组与CCL21(100ng/mL)组中穿膜细胞数分别为(48±4)个和(113±7)个,差异有统计学意义(P<0.05);CCL21(100ng/mL)组中MMP-9的相对表达水平为0.83±0.02,高于阴性对照组的0.38±0.01(P<0.05)。CCL21单独作用不能促进SW480细胞增殖;VP-16组中SW480细胞的增殖抑制率为68.3%,CCL21预处理增强SW480细胞活力,CCL21(100ng/mL)中抑制率降至47.4%;VP-16组与CCL21(100ng/mL)组中凋亡率分别为(65.2±5.2)%和(48.7±3.1)%,差异有统计学意义(P<0.05)。结论:CCL21/CCR7可以促进结肠癌SW480细胞的体外侵袭力,并增强其在微环境中的生存能力。
Background and Objective： Chemokine receptor CCR7 is upregulated in gastrointestinal carcinomas and is significantly associated with lymphatic invasion and lymph node metastasis. This study was to investigate the role and mechanism of CCL21/CCR7 in invasion of colorectal carcinoma cell line SW480. Methods： The invasive capacity of SW480 cells was examined using Wound healing assay and Transwell assay. Expression of matrix metalloproteinase-9 （MMP-9） was measured by Western blot. SW480 cells were pre-incubated with CCL21 for 2 h before exposure to VP-16 （20 ng/mL）. Cell proliferation was measured using MTT assay. Cell apoptosis was analyzed by flow cytometry and Hoechst33258 staining. Results. Compared to the control group, more cells in the CCL21 treatment group migrated into the gap at same time points; the count of SW480 cells penetrating through the membrane after the treatment of 100ng/mL CCL21 increased significantly [（113±7） vs. （48±4）] （P〈0.05）. and the relative expression of MMP-9 in the CCL21 treatment group was enhanced evidently [（0.83±0.02） vs. （0.38±0.01）J （P〈0.05）. Although CCL21 alone did not promote proliferation of SW480 cells, pre-incubation of cells with 100ng/mL CCL21 attenuated the inhibitory effect of VP-16 on proliferation of SW480 from 68.3% to 47.4%, and reduced the apoptotic rate from （65.2±5.2）% to （48.7±3.1）%. Conclusion： CCL21 enhances the invasive ability of SW480 cells, induces MMP-9 expression, and promotes the survival of SW480 cells under the suboptimal circumstance in vitro.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2009年第7期708-713,共6页
Chinese Journal of Cancer
基金
国家自然科学基金项目(No.30772128)~~
