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茶树咖啡碱生物合成相关酶基因表达研究 被引量:3

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摘要 用半定量PCR法,分别研究了4个月幼龄茶树嫩叶、成熟叶片、茎、根以及愈伤组织中咖啡碱合成酶(TCS1)、次黄嘌呤苷-5′-单酸磷酸脱氢酶(TIDH)、s-腺苷甲硫氨合成酶(sAMS)等咖啡碱生物合成相关酶基因的差异表达。研究表明,茶树嫩叶中TCS1基因表达量高于其他部位,TIDH基因叶内表达量多于茎和根中,但sAMS表达量在茶树各个部位差异不大;在茶树愈伤组织中,幼嫩组织TCS1基因表达量也远高于老化组织。这些结果支持前人酶学与代谢学研究中咖啡碱主要在嫩叶中合成的结论,可以推测嫩叶中咖啡碱的生物合成取决于咖啡碱合成酶的较强表达。
出处 《中国茶叶》 2009年第6期20-21,共2页 China Tea
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  • 1Ashihara H , Sano H, Crozier A. Caffeine and related purine alkaloids: Biosynthesis,catabolism, function and genetic engineering[J]. Phytochemistry, 2008,69( 4 ): 841-856. 被引量:1
  • 2Ashihara H, Kubota H. Patterns of adenine metabolism and caffeine biosynthesis in different parts of tea seedling[J]. Physiol. Plant, 1986( 68 ): 275-281. 被引量:1
  • 3Kato M, Mizuno K, Fujimura T, et al. Purification and characterization of caffeine synthase from tea leaves[J]. Plant Physiol, 1999( 120 ): 597-586. 被引量:1
  • 4Kato M, Mizuno K, Crozier A, et al. A gene encoding caffeine synthase from tea leaves[J]. Nature, 2000 (406): 956-957. 被引量:1
  • 5冯艳飞,梁月荣.茶树S-腺苷甲硫氨酸合成酶基因的克隆和序列分析[J].茶叶科学,2001,21(1):21-25. 被引量:45
  • 6Keya C A, Crozier A, Ashihara H. Inhibition of caffeine biosynthesis in tea ( Camellia .sinensis ) and coffee ( Coffea arabica ) plants by ribavirin[J]. FEBS Lett, 2003(554 ) 473-477. 被引量:1
  • 7Keya C A. Biosynthesis of useful nitrogen compounds in tea plants ( Camellia sinensis ) and its exploitation in biotechnology[D]. Tokyo: Ochanomizu University, 2004. 被引量:1
  • 8Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures[J]. Physiol. Plant, 1962 ( 15 ): 473-497. 被引量:1
  • 9Mira, Gallo A, Greco V, Zasiura C, et al. Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis ) seed[J]. Eur. J. Biochem, 2001 ( 268 ):1500-1507. 被引量:1
  • 10Ashihara H,Crozier A. Biosynthesis and metabolism of caffeine and related purine alkaloids in plants[J].Adv. Bot. Res., 1999( 30): 118-205. 被引量:1

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  • 1张华艳,戚丽,张正竹.咖啡碱对茶树主要叶部病原真菌的抑制作用[J].南京农业大学学报,2010,33(2):63-67. 被引量:14
  • 2张广辉,梁月荣,陆建良,董俊杰.茶树咖啡因合成酶基因RNA干涉表达载体构建[J].茶叶科学,2006,26(4):243-248. 被引量:19
  • 3王荣焕,王天宇,黎裕.关联分析在作物种质资源分子评价中的应用[J].植物遗传资源学报,2007,8(3):366-372. 被引量:40
  • 4Ashihara H, Suzuki T. 2004. Distribution and biosynthesis of caffeine in plants. Frontiers in Bioscience, 9.. 1864 - 1876. 被引量:1
  • 5Chu Y G, Su X H, Huang Q J, Zhang X H. 2009. Patterns of DNA sequence variation at candidate gene loci in black poplar (Populus nigra L. ) as revealed by single nucleotide polymorphisms. Genetica, 137:141 - 150. 被引量:1
  • 6Flint-Garcia S A, ThuilletAC, Yu J M, Pressoir G, Romero S M, Mitchell S E, Doebley J, Kresovich S, Goodman M M Maize association population: A high-resolution platform for quantitative trait locus dissection. The Plant Journal, 44(6): Buckler E S. 2005 1054 - 1064. 被引量:1
  • 7Guillet-Claude C, Birolleau-Touchard C, Manicacci D, Fourmann M, Barraud S, Carret V, Martinant J, Barrire Y. 2004. Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis. Theoretical and Applied Genetics, 110 (1): 126 - 135. 被引量:1
  • 8Gupta P K, Rustgi S, Kulwal P L. 2005. Linkage disequilibrium and association studies in higher plants: Present status and future prospects. Plant Molecular Biology, 57.. 461-485. 被引量:1
  • 9HillW, bcrtsnA.96.Linkagdisequiibriuminnitppuatins.ThercticaandAppidGcntics 38(6): 226-231. 被引量:1
  • 10Hudson R R, Kaplan N L 1985. Statistical properties of the number of recombination events in the history of a sample of DNA sequences. Genetics, 111.. 147 - 164. 被引量:1

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