摘要
[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA sequence of CYP305 B1 V1 Gene published in GenBank for wild mulberry silkworm, one pair of primers was designed, and the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm treated by NaF, rutin, cypermethrin and ecdysone was also analyzed by the semi - quantitative RT - PCR. Furthermore, homology comparison and phylogenetic analysis for amino acid sequences of this gene were studied. [ Result] Rutin, cypermethrin and NaF had effects on the expression of P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm, while ecdysone had no significant effect. Homology comparison for amino acids indicated that the amino acid sequence of this gene was the most similar to that of CYP305 B1 gene in Bombyx mori with 100% amino acid identity, and highly similar to those of Tribolium casmneum CYP305A1, Apis mellifera CYP305A1, Drosophi- la melanogaster CYP305A1, Anopheles gambiae CYP305A2and Culex pipiens quinquefasciatus CYP2LI. [ Conclusion] CYP305 B1 V1 Gene of wild mulberry silkworm is likely to mainly take part in the metabolism of exogenous compounds, which is of great significance for revealing the function of cytochrome P450 and the metabolic mechanism of different drugs.
1材料与方法1．1材料与试剂野桑蚕采自苏州大学独墅湖校区桑园；宿主菌E．coli TOP10为苏州大学基础医学及生物科学学院生物资源与功能基因组学研究室保存；植物次生性物质芸香苷和内源性物质蜕皮激素均购自SIGMA公司；氯氰菊酯购自拜耳杭州作物科学有限公司；外源性化合物NaF（分析纯）购自西安化学试剂厂；RNAiso reagent试剂盒购自TakaRa公司；其他常用试剂购自TaKaRa公司或上海生工生物工程技术服务有限公司：
基金
国家973计划项目(2005CB121005)资助~~
