摘要
目的探讨宫内缺氧对新生大鼠海马CA3区血管内皮细胞生长因子(VEGF)表达与胶质细胞分化的影响以及当归的调控作用。方法选择雌性SD大鼠分别与雄性大鼠合笼。孕第14天健康SD雌性大鼠15只随机分为对照组、缺氧组和当归干预组,每组各5只。自孕第14天开始将当归干预组与缺氧组孕鼠置于低张氧三气培养箱中,制作胎鼠宫内缺氧模型,此前1h按8mL/kg分别予250g/L当归注射液和9g/L盐水尾静脉注射,对照组不予缺氧处理。3组孕鼠分娩当日每窝随机选取新生大鼠4只,取其脑组织多聚甲醛固定,石蜡包埋切片,胶质纤维酸性蛋白(GFAP)mRNA、VEGFmRNA原位杂交,400倍拍照。结果缺氧组新生大鼠海马CA3区GFAPmRNA与VEGFmRNA阳性细胞的积分吸光度(A)值均较对照组增大(Pa<0.05);当归干预组GFAPmRNA的A值较缺氧组减小(P<0.05),VEGFmRNA的A值较缺氧组增大(P<0.05)。结论一定时间、一定氧体积分数的宫内缺氧可刺激新生大鼠海马CA3区胶质细胞的增生,其机制可能与缺氧后导致新生大鼠海马CA3区VEGFmRNA表达增高有关;当归注射液可减弱宫内缺氧新生大鼠神经胶质细胞的增生,可能是通过上调VEGFmRNA的表达而改善缺氧环境所致。
Objective To explore the effect of intrauterin hypoxia on the expressions of vascular endothelial growth factor（VEGF） and gliocyte differentiation in hippocampal CA3 area of neonatal rats following in tranterinhypoxia, and the regulative role of angelica on it. Methods Female adult SD rats weighted 220 - 250 g, separately, mate with male rat. Fifteen pregnant healthy SD rats were divided randomly into 3 groups： control group,hypoxia group and angelica group, each group contained 5 rats. Angelica group and hypoxia group pregnant rats were placed in the three - gas incubator from the beginning of pregnant 14^th day to produce the intrauterine hypoxia model of fetal rats, and 1 hour ago, the pregnant rats of the 2 groups received injections respectively 250 g/L angelica injection and normal saline（8 mL/kg） through the caudal vein of rats. The control group procedures were identical to the hypoxia group, but under normal, non - hypoxic conditions. After birth ,4 neontal rats were selected randomly from each litter to take the brain tissue, to be fixed in paraformaldehyde, embedded in paraffin and sectioned. Glial fibrillary acidic protein（GFAP） mRNA and VEGF mRNA in situ hybridization. The photos in hippocampal CA3 area were taken under microscope （ × 400）. Results The integral absorbance values of positive GFAP mRNA and VEGF mRNA cells in hypoxia group were obviously higher than those of control group （Pa 〈 0.05 ）, and the absorbance value of positive GFAP mRNA cells in angelica group was significantly lower than that of hypoxia group （P 〈 0. 05 ）, but the absarbance value of positive VEGF mRNA cells in angelica group was higher than that of hypoxia group （ P 〈 0.05 ）. Conclusions Intrauterine hypoxia in a certain time and a certain degree of oxygen concentration can stimulate gliocyte of hippocampal CA3 area to proliferate, and the mechanism is related possibly with high expression of VEGF mRNA after intrauterine hypoxia,but angelica injection can weaken gliocyte proliferation ,
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第12期921-923,共3页
Journal of Applied Clinical Pediatrics
基金
四川省科技厅课题资助(05JY029-103)
四川省卫生厅课题资助(060052)
