摘要
目的探讨当归注射液对宫内缺氧胚胎大鼠神经干细胞增殖的影响。方法将孕19天孕鼠经尾静脉注入当归注射液,用低张性缺氧模型致鼠胚宫内缺氧,将鼠胚大脑组织作神经巢蛋白免疫组化染色后,进行图像分析。结果缺氧组鼠胚Nestin免疫组化反应较对照组增强,阳性细胞增多(P<0.05);而当归组鼠胚较缺氧组减弱,阳性细胞减少(P<0.05)。结论缺氧可刺激神经干细胞进入增殖状态;当归注射液对宫内缺氧大鼠神经干细胞可能具有保护作用;采用低张性缺氧方法制造胚胎宫内缺氧模型。
[Objective] To study the effect of Angelica injection on proliferation of neural stem cells（NSCs） from embryonic rats of hypoxia in the inner of the uterus. [Methods] The conceiving female rats were injected with Angelica injection through caudal vein before their embryos were in hypotonie hypoxia in the inner of the uterus. The expression of Nestin in brain of embryos was studied with immunohistochmistry, then the results were analyzed by image processing system. [Result] The colour of the immunohistochemical staining of Nestin in hypoxia group（HG） was darker than that of control group （CG） （P 〈0.05）, and the quantity of postive cells in HG was more than that of CG （P 〈0.05）. But in Angelica group （AG）, them were lighter and less than that of HG（P 〈0.05）. [Conclusion] Hypoxia can stimulate the proliferation of the NSCs. Angelica injection could play a protective role to NSCs from embryonic rats in hypoxia. The model of hypoxia in the inner of the uterus could be established by making the con- ceiving female rat in hypotonic hypoxia.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第21期3226-3228,共3页
China Journal of Modern Medicine
基金
四川省教育厅资助课题(No:2001149)
关键词
当归
缺氧
千细胞
增殖
angelica
hypoxia
stem cell
proliferation
