摘要
目的:探讨大鼠一侧睾丸扭转后行切除术对另侧睾丸组织的保护作用。方法:将63只青春期前SD大鼠随机分为假手术组、扭转复位组和扭转切除组,每组21只。假手术组将左侧阴囊打开分离暴露睾丸后,不予扭转,即将睾丸再固定于阴囊壁上,并缝合阴囊。扭转复位组和扭转切除组则依Turner法建立左侧睾丸扭转模型,均先扭转4h,其后前者行复位固定术,后者行切除术。三组分别于复位后4h、24h、1周各处死大鼠7只,取右侧睾丸行丙二醛(MDA)、白介素-6(IL-6)检测及病理评估,左侧睾丸仅行病理评估。结果:各组左侧睾丸病理评分值均达到最差分数,病理切片结果示存在绝大部分坏死或完全性坏死。与扭转切除组比较,扭转复位组对侧睾丸的MDA、IL-6值、病理评分等均有不同程度升高,且1周时生精细胞层数结合精细小管直径所示的预后情况亦较差;而各指标在扭转切除组与假手术组的相比中未示明显差异。结论:单侧睾丸扭转复位后对侧睾丸早期即可出现继发性损伤。在明确扭转侧睾丸出现坏死后,不复位直接行切除术可以保护对侧睾丸。
Objective： To explore the protective effects of orchiectomy on contralateral testis of SD rats with unilateral testicular torsion through the biochemical and histological indexes. Methods ： Sixty-three prepubertal Sprague-Dawley rats were randomly divided into 3groups, with 21 rats in each group. The shun operation group underwent a sham operation of the left testis under general anaesthesia. The torsion/detorsion group and the torsion/orchiectomy group underwent a similar operation but the left testis was rotated 720 o clockwisely for 4h, maintained by fixing the testes to the scrotum, then detorsion was performed in the torsion/detorsion group,while orchiectomy was performed in the torsion/orchiectomy group. Both testes of seven rats from each group were sacrificed at 4 hour, 24 hour and l week intervals after surgery. Then, the biochemical parameters such as malondialdehyde （MDA） and interleukin-6 （IL-6） were evaluated; histology index and seminiferous tubule diameter were assessed on right testes, while the left were only assessed by histology index. Results：Compared with The torsion/orchiectomy group, the biochemical parameters and histological grades in the torsion/detorsion group increased in different degrees, and the prognosis status demonstrated by the number of spermatogenic cell associated with the decreased mean diameter of the seminiferous tubule, there are a worse prognoses in the torsion/detorsion group. However, there were no significant differences of every indexes between the sham operation group and the torsion/ orchiectomy group. It＇ s also known that all the left testes get the most vicious histological grade, and pathological sections showed completely necrosis or severe bulk necrosis. Conclusion：These results suggest that the injuryofcontralateral testes may occur in early time after unilateral testiculartorsion/ detorsion, and whenever, the testis necrosis definitely, performed orchiectomy after unilateral testicular torsion without detorsion can do protective effects on
出处
《温州医学院学报》
CAS
2009年第3期209-212,共4页
Journal of Wenzhou Medical College
关键词
一侧睾丸扭转
对侧睾丸损伤
睾丸切除术
保护
unilateral testicular torsion
contralateral testis injury
orchiectomy
protection
