摘要
目的:探讨5型磷酸二酯酶抑制剂他达拉非在大鼠睾丸缺血再灌注模型中的保护作用。方法:将84只健康成年雄性SD大鼠随机分成4组,A组:假手术组;B组:小剂量他达拉非组;C组:大剂量他达拉非组;D组:安慰剂组。每组21只,B、C、D组建立右侧睾丸扭转复位模型(720°,2 h)。术后立即予A、B组按他达拉非剂量为0.5 mg/(kg·d)灌胃,C组按他达拉非剂量为2 mg/(kg·d)灌胃,D组用等剂量的生理盐水灌胃,用药3、7、14 d后,取各组7只大鼠扭转侧睾丸,检测睾丸组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并光镜检测睾丸组织病理学参数。结果:B组[3 d:(254.46±7.43)n U/mg prot;7 d:(278.49±8.33)n U/mg prot;14 d:(317.99±3.31)n U/mg prot]、C组[3 d:(277.12±8.80)n U/mg prot;7 d:(309.40±2.14)n U/mg prot;14 d:(320.39±4.72)n U/mg prot]SOD活性均显著高于同期D组[3 d:(223.21±4.65)n U/mg prot;7 d:(231.45±4.16)n U/mg prot;14 d:(248.28±5.74)n U/mg prot],MDA含量均显著低于同期D组。C组3、7 d的SOD活性显著高于同期B组(P<0.01),而MDA含量显著低于同期B组(P<0.01),14 d时的SOD与MDA两组间没有显著性差异(P>0.05)。睾丸组织的病理学改变:A组睾丸组织未见明显损伤,B组3、7、14 d之间病理学参数(生精上皮层数、睾丸结构得分、生精小管直径)存在显著差异(P<0.01),B组14 d时和C组7 d时测得的生精上皮层数、睾丸结构评分、生精小管直径与A组14 d时没有显著性差异。结论:他达拉非可明显改善睾丸扭转后的缺血再灌注损伤,改善程度与用药时间、用药剂量呈明显的相关性。
Objective： To investigate the protective effect of phosphodiesterase type 5 inhibitors（ tadalafil） on the testis following testicular ischemia-reperfusion injury in rats. Methods： Eighty-four healthy adult male SD rats were randomly and equally divided into groups A（ sham operation）,B（ testicular torsion ＋ low-dose tadalafil）,C（ testicular torsion ＋ high-dose tadalafil）,and D（ testicular torsion ＋ placebo）. Models were established in the latter three groups by 720° torsion of the right testis for 2 hours. The animals in groups A and B were treated by gavage with tadalafil at the dose of 0. 5 mg per kg per day,those in group C at 2 mg per kg per day,and those in group D with saline at the same dose. After 3,7,and 14 days of treatment,the torsioned testes were harvested for evaluation of the superoxide dismutase（ SOD） activity and malondialdehyde（ MDA） content in the testis tissue. The pathological changes in the testis were observed under the light microscope. Results： At 3,7,and 14 days,the SOD activity was（ 254. 46 ±7. 43）,（ 278. 49 ± 8. 33）,and（ 317. 99 ± 3. 31） n U / mg prot in group B,and（ 277. 12 ± 8. 80）,（ 309. 40 ± 2. 14）,and（ 320. 39± 4. 72） n U / mg prot in group C,all obviously higher than in D（ [223. 21 ± 4. 65],[231. 45 ± 4. 16]and [248. 28 ± 5. 74]n U / mg prot）,while the MDA content was lower in the former two groups than in the latter. At 3 and 7 days,the SOD activity was significantly higher and the MDA level significantly lower in group C than in B（ both P 〈 0. 01）,while at 14 days,neither showed any remarkable differences between the two groups（ P 〉 0. 05）. No obvious histopathological change was observed in the testis tissue of group A. At3 and 7 days,pathological examination of the testis tissue revealed significant differences in the number of seminiferous epithelial layers,testicular histological score,and seminiferous tubule diameter in group B（ P 〈 0. 01）,but the three indexes at 14 days in grou
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第3期214-218,共5页
National Journal of Andrology
基金
温州市科技局课题(Y20110166)~~
关键词
睾丸扭转
再灌注损伤
5型磷酸二酯酶抑制剂
他达拉非
testicular torsion
ischemia-reperfusion injury
phosphodiesterase type 5 inhibitor
tadalafil
