摘要
目的了解转录因子Sp1、骨桥蛋白OPN在不同转移潜能的人大肠癌细胞株中的表达。方法以体外建系的大肠癌细胞株SW480、SW620为研究对象,通过Real-time PCR、Western-blot、细胞免疫荧光化学染色方法从mRNA和蛋白水平分别检测Sp1、OPN的表达。结果Real-time PCR的结果提示SW480(Duke’s B期)、SW620(Duke’s C期)两株细胞株都表达Sp1mRNA、OPN mRNA,Western-blot检测Sp1、OPN蛋白在两种大肠癌细胞株中均有表达,SW620细胞株Sp1、OPN表达水平高于SW480(P<0.05)。结论Sp1、OPN在大肠癌细胞株SW480、SW620中均有表达,与大肠癌肿瘤分期、侵袭转移相关。
Objective To explore the expression of transcription factor Spl and osteopontin （OPN） in the colorectal carcinoma cell lines （SW480 and SW620） with different metastatic potential. Methods Taken established human colorectal cancer cell lines SW480 and SW620 in vitro as research object, Real-time PCR, Western-bolt methods and cellular immunofluorescence technique were used to detect the Sp1. OPN expression at gene level and protein expression level. Results Real-time PCR results showed SW480 （Dukes B stage） and SW620 （Dukes C stage） had Sp1 and OPN expression; Western-bolt also showed the expression of Sp1 and OPN protein existed in both cell lines. The level of Spl and OPN expression in cell line SW620 was higher than in SW480 （P〈0.05）. Conclusion The expression of Sp1, OPN were observed in the colorectal carcinoma cell lines （SW480 and SW620）, and were significantly correlated to the tumor invasion and Dukes stages.
出处
《福建医药杂志》
CAS
2009年第3期65-68,共4页
Fujian Medical Journal
关键词
大肠癌
骨桥蛋白
转录因子SP1
Colorectal carcinoma
Osteopontin （OPN）
Transcription factor Sp1
