摘要
目的:探讨急性心肌梗死(AMI)患者血清瘦素(Leptin,LP)水平的变化及外周血单个核细胞内皮素受体(ETA-R)mRNA的表达和临床意义。方法:采用酶联免疫法测定65例AMI患者和70例健康对照组血清LP水平;逆转录聚合酶链反应(RT-PCR)测定ETA-RmRNA的表达。结果:AMI患者在<6h、>6h、24h采血,LP水平随时间的延长而增高,24h达到高峰,48h后反而下降。6h内与48h组LP水平与对照组相比,差异有统计学意义(P<0.05);>6h、24h组与对照组相比,差异有统计学意义(P<0.01);6h内与48h组相比差异无统计学意义(P>0.05)。AMI患者ETA-RmRNA的表达明显增高,随时间的延长变化不明显,维持在一个较高的水平。与对照组相比,差异有统计学意义(P<0.01);AMI组内相比差异无统计学意义(P>0.05)。经直线相关分析:LP水平与ETA-RmRNA的表达无相关性(P>0.05)。结论:AMI患者血清LP水平及ETA-RmRNA的表达明显升高,分别是AMI发生的重要的独立危险因素及预测因子,早期监测LP水平及ETA-RmRNA的表达,有利于AMI的预防。
Objective： To explore serum leptin levels and expression of endothelin A receptor（ETA-R） mRNA in peripheral blood monocytes in patients with acute myocardial infarction（ASMI）. Methods： The level of serum LP was assayed by enzyme-linked immunosorbent assay in 65 cases with AMI and 70 healthy cases in control group. The expression of ETA-R mRNA was assayed by RT-PCR. Results： Compared with control group, there was significant increase in serum LP levels at 〈 6h and 48h in AMI group （P 〈 0.05 ） ; there was significant difference at 〉6h and 24h in AMI group（P〈0.01 ）, and there was no significant difference at 〈 6h and 48h in AMI
group （ P 〉 0.05 ）. The expression of ETA-R mRNA was significantly increased in AMI patients, remaining a high level （P 〈0.01 ）. Linear correlation analysis showed that there was no correlation between LP and ETA-R mRNA expression （P〉 0.05）. Conclusion： The serum LP levels and ETA-R mRNA expression were significantly increased in AMI patients, and they are important risk and predictive factors for AMI patients. Monitoring the level of the LP and ETA-R mRNA expression as early as possible is favorable to the prevention of AMI.
出处
《海南医学院学报》
CAS
2009年第7期702-704,共3页
Journal of Hainan Medical University
基金
海南省自然科学基金项目(808234)~~
