摘要
目的观察补充β-胡萝卜素对大鼠急性放射性损伤的保护作用。方法取40只雌性SD大鼠,随机分为正常对照组（A组）、阳性对照组（B组）、β-胡萝卜素5 mg/（kg·d）组（C组）、β-胡萝卜素10 mg/（kg·d）组（D组）。照射前C组按5 mg/（kg·d）灌喂β-胡萝卜素,D组按10 mg/（kg·d）灌喂β-胡萝卜素,A组及B组按照相同体积灌喂花生油。连续灌胃14 d,第15天除A组外,其余各组大鼠分别给予腹部单次剂量9 Gy一次性X线照射。4 d后处死大鼠,检测各组大鼠血浆超氧化物歧化酶（SOD）、丙二醛（MDA）含量及谷胱甘肽过氧化物酶（GSH-PX）活性。用单细胞凝胶电泳测定各组大鼠DNA损伤情况。结果与B组比较,C、D组大鼠血浆MDA含量明显下降（F=5.23,q=4.20、2.80,P〈0.05）。D组大鼠血浆GSH-PX活性较B组明显升高（F=3.70,q=5.11,P〈0.05）。0μmol/L H2O2诱导淋巴细胞氧化损伤时,B组大鼠淋巴细胞DNA自发性损伤情况较其他3组严重（F=105.06,q=19.85-21.53,P〈0.05）。结论补充一定剂量β-胡萝卜素对X线腹部放射引发的生物膜脂质过氧化及DNA氧化损伤有较好的防护作用。
Objective To study the protection of β-carotene （β-C） on rat with acute radiation damage. Methods Forty female SD rats were randomized to four groups： normal control group （A）, positive control groups（B）, 5 mg/（kg ·d）β-C group （C） and 10 rag/ （kg ·d） β-C group （D）. Before radiation, 5 mg/（kg ·d） of β-C was given to rats in group C, 10 mg/（kg ·d） of β-C to those in group D, and same volume of peanut oil was given to those in groups A and B. The intragastric administration lasted for 14 days. On 14th day, a single abdominal radiation of 9 Gy was offered to all the rats, except those in group A. The rats were sacrificed four days later, plasma superoxide dismutases （SOD）, malondialdehyde （MDA）, and glutathion peroxidase （GSH-PX） were detected and damage of DNA was checked using single cell gel electrophoresis technique. Results Compared with group B, the contents of MDA in groups C and D were significantly lower （F= 5.23;q= 4.20,2.80;P〈0.05） ; the activity of GSH-PX in group D was higher than that of group B （F=3.70;q=5. 11;P〈0. 05）. The damage of DNA in group B was the most severe corn pared with the other three groups （F= 105.06;q= 19.85-21.53;P〈0.05）. Conclusion Supplement of a certain dose of β-C will have a better protection on lipid peroxidation and oxidative damage of DNA induced by radiation injury of the abdomen.
出处
《青岛大学医学院学报》
CAS
2009年第5期416-418,共3页
Acta Academiae Medicinae Qingdao Universitatis
