摘要
本试验建立了测定动物源性食品中敌菌净残留量的高效液相色谱法。样品用碱性二氯甲烷提取并经浓缩后,在酸性条件下用正己烷、乙酸乙酯和阳离子交换小柱净化。固体提取物溶解在0.1mol/L盐酸溶液中,用配有紫外检测器的高效液相色谱仪测定,外标法定量。敌菌净线性范围为0.01-2.5μg/mL,其相关系数和回归方程分别为r=0.99986,y=13.3808 x-0.1409;样品平均加标回收率为87.8%-97.1%,相对标准偏差为2.73%-7.32%;方法的最低检出限为0.01mg/kg。
A high performance liquid chromatographic method （HPLC） was developed for the determination of diaveridine residues in animal derived food. After the extracts are evaporated, the sample is cleaned up with n -hexane and ethyl acetate, and cation exchange column under acidic condition. The extract is dissolved with 0. 1moL/L HCL and determined by HPLC with VWD -detector, using external standard method. The liner range was 0.01μg/mL - 2.5μg/mL. The correlation coefficients were 0.99986. The regression equation was y = 13. 3808 x -0. 1409. The average recoveries of diaveridine in spiked samples ranged from 86.3 - 101.5% , and the relative standard deviations were between 1.81% - 7.16%. The detection limit was 0.01mg/kg.
出处
《检验检疫科学》
2009年第3期27-29,共3页
Inspection and Quarantine Science
