摘要
为摸索大鼠蜕膜细胞的培养方法,采用胰蛋白酶消化蜕膜组织,全组分蜕膜细胞培养,传代提纯蜕膜基质细胞,在4孔细胞培养板中直接进行泌乳素(PRL)免疫细胞化学检测培养细胞的组成。所采用的方法具有耗时少、细胞活率高的优点。结果:实验方法简单、经济、实用。
To investigate the way of rat decidual stormal cells Cuhureed in vitro, the following methods were adopted ： Trypsin was used to digeste the rat decidual tissue. All components of the decidual cells were recovered. PRL immunohistochemistry assay was employed to testify the components of the cultured cells in a cell - cultured plate. The results demonstrated that the method had the advantages of shorter time consumption and high survival cell rate.
出处
《贵州畜牧兽医》
2009年第3期9-10,共2页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
关键词
SD大鼠
蜕膜细胞
培养
SD rats
Decidual stromal cells
Culture
