摘要
应用L9(34)正交设计优化碱性蛋白酶水解小麦面筋蛋白的最适条件,以DPPH自由基清除率为筛选指标,结果表明当温度为60℃、pH 8、底物量5 g、水解时间25 min时,酶解液的DPPH自由基清除率达到87.67%,具备较强的体外抗氧化活性,值得深入研究。
Based on the results of DPPH radical scavenging activities of the hydrolyzates, the enzymatic hydrolysis conditions of wheat gluten by Alcalase was optimized with the orthogonal design L9（3^4）. Results obtained showed that the optimal conditions of Alcalase to hydrolyze the wheat gluten could be set as： 60 ℃, pH 8, substrate 5 g, and hydrolysis time 25 min. Under above conditions, DPPH radical scavenging ratio of the hydrolyzates reached to 87.67% Under the optimal conditions, the hydrolyzates of wheat gluten by Alcalase have good antioxidant activity in vitro and are valuable enough for the future research.
出处
《食品科技》
CAS
北大核心
2009年第6期153-156,共4页
Food Science and Technology
基金
国家高技术研究发展计划资助项目(863计划项目)(2007AA10Z323)
江苏省教育厅自然科学基金(07KJD350034)
江苏大学高级人才基金资助项目(07JDG020)
