摘要
目的研究L-肌肽防治大鼠硒性白内障的作用。方法实验研究。用亚硒酸钠制作大鼠白内障模型，分为3组，每组9只，分别用50g/L、20g/L的L-肌肽滴眼液及生理盐水滴眼3周，观察大鼠晶状体变化情况。将大鼠白内障晶状体体外培养，分别加入1．00、0．10及0．01g／L的L-肌肽无血清营养液，培养1周，观察大鼠晶状体变化。再将培养的大鼠白内障晶状体进行双向电泳实验。采用方差分析对各组间的均数进行比较。结果大鼠用药1周，50g／L、20g／L的L-肌肽组和对照组的晶状体病变度数分别为2．22±0．65、2．39±0．98及2．83±0．38；50g／L的L-肌肽组病变轻于对照组，差异有统计学意义（P=0．013）。用药2周和3周，3组间晶状体病变差异无统计学意义（P〉0．05）。大鼠白内障晶状体培养1周，1．00、0．10及0．01g/L的L-肌肽组晶状体病变与对照组差异无统计学意义（P〉0．05）。双向电泳结果显示：药物组蛋白总数为182，对照组为161，药物组高相对分子质量蛋白数量减少，低相对分子质量蛋白数量增多。结论50g／L的L-肌肽可抑制大鼠白内障早期病变的发展；肌肽在体外可使大鼠白内障晶状体蛋白发生变化，对白内障晶状体病变程度作用不观显。
Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops （50 g/L or 20 g/L ） and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L- canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22± 0.65, 2.39 ± 0.98 and 2.83 ± 0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/L L-canosine group was lighter than that of the control group. There was significant difference between these two groups （ P = 0. 013）. On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for cuhuring cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L- carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-carnosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2009年第6期533-536,共4页
Chinese Journal of Ophthalmology
基金
北京市科技计划项目（Y0204002041331）
关键词
肌肽
大鼠
亚硒酸钠
白内障
免疫电泳
双向
Carnosine
Rats
Sodium selenite
Cataract
Immunoelectrophoresis, two- dimensional
