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川渝产山银花中总黄酮和总绿原酸的含量测定 被引量:2

Determination of total flavonoids and total chlorogenic acid in Flos Lonicerae produced in Sichuan and Chongqing areas
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摘要 目的:建立川渝产山银花中总黄酮和总绿原酸的含量测定方法。方法:采用UV法测定总黄酮和总绿原酸。结果:总黄酮在0.02~0.08 mg/ml范围内具有良好的线性关系(R=0.9998),回收率为98.15%,RSD为0.45%。总绿原酸在0.8~8μg/ml范围内具有良好的线性关系(r=0.9998),平均回收率为98.75%,RSD为0.29%。结论:该方法操作简便、快速、有效、灵敏、准确、具有良好的重复性和回收率,可作为山银花总黄酮和总绿原酸的含量测定方法。 Objective:To establish the method for content determination of total flavonoids and total chlorogenic acid in Flos Lonieerae produced in Siehuan and Chongqing areas. Methods:The content of total flavonoids and total chlorogenic acid was detected by UV. Results :The calibration curve of total flavonoids showed good linearity over the range of 0.02-0.088mg/ml (R=0.9998). The average recovery was 98.15% with RSD 0.45%. The calibration curve of total chlorogenic acid showed good linearity over the range of 0.8-8μg (r=0.9998). The average recovery was 98.75% with RSD 0.29%.Conclusion :This method is simple ,rapid,efficient, sensitive and accurate with good repeatability and recovery. It can be used as a quantitative analysis method for this preparation.
出处 《现代医药卫生》 2009年第14期2087-2088,共2页 Journal of Modern Medicine & Health
关键词 川渝产山银花 总黄酮 总绿原酸 含量测定 Flos Lonicerae produced in Sichuan and Chongqing areas Total flavonoids Total chlorogenic acid Content determination
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