摘要
应用免疫组化ＬＳＡＢ法，对５０例原发性肝癌、癌周肝组织及１０例正常肝组织中ｃ－ｅｒｂＢ－２、Ｈ－ｒａｓｐ２１和ｐ５３癌基因蛋白及ＰＣＮＡ表达进行比较研究。结果表明，ｃ－ｅｒｂＢ－２、Ｈ－ｒａｓｐ２１、ｐ５３及ＰＣＮＡ阳性表达率，在癌组织中分别为７０．０％、６８．０％、４６．０％及８２．０％；在癌周肝组织中分别为２２．０％、６４．０％、４．０％及５２．０％；正常肝组织中（除Ｈ－ｒａｓｐ２１外）未见表达，且癌组织与非癌组织（Ｈ－ｒａｓｐ２１除外）及正常肝组织之间有显著差异（Ｐ＜０．０５）。ｃ－ｅｒｂＢ－２和ｐ５３蛋白的表达与原发性肝癌的组织学分级有密切关系（Ｐ＜０．０５），且二者的表达有高度的相关性（Ｐ＜０．００５），在癌、癌周肝组织中，２种以上癌基因蛋白同时表达总率分别为１４４．０％、１２．０％，癌基因蛋白与ＰＣＮＡ同时表达总率分别为１５０．０％、８４．０％。提示肝癌的发生是由多种癌基因独立并协同作用伴有细胞异常增殖的恶性转化过程。
The expressions of c erbB 2、H ras p21、p53 oncogene protein and PCNA in tumor tissues,peritumor tissues of 50 cases of primary hepatic carcinoma (PHC) and normal liver tissues of 10 cases of control were detected by immunohistochemistry LSAB method.The results showed that the positive rates of c erbB 2、H ras p21、p53 and PCNA expression were 70.0%,68.0%,4.0%,and 82.0% in tumor tissues and 22.0%,64.0%,4.0%,and 52.0% in peritumor tissues,respectively.They were negative (except H ras p21)in normal tissues. There were notable differences between the tumor tissues and peritumor tissues(H ras p21) and normal liver tissues ( P <0.05).The positive rate of c erbB 2 or p53 was found to be closely associated with histologic grade in PHCs ( P <0.05);And the c erbB 2 expression was closely correlated to the p53 expression in PHCs ( P <0.05).Co expression of two or three oncogenes occurred in 144.0% of tumor tissues,12.0% of peritumor tissues.The co expressions of the two oncogenes binding PCNA occurred in 150.0% of PHCs,84.0% of peritumor tissues.It is suggested that the hepatocarcinogenesis and development is related to various oncogenes,in simple or combine way,simultaneously implicated the malignant transformation process with cellular aberrant proliferation.
出处
《实用癌症杂志》
1998年第2期98-101,共4页
The Practical Journal of Cancer
关键词
肝肿瘤
原发性
C-ERBB-2
增殖细胞核抗原
表达
c erbB 2 H ras p21 p53 Proliferatiing cell nuclear antigen Primary hepatic carcinoma Expression
