摘要
目的克隆豚鼠钙调蛋白1(CaM1)基因,对所克隆基因进行生物信息学分析。方法从豚鼠心室肌组织提取总RNA,应用RT-PCR方法扩增CaM1基因编码区447bp的cDNA片段,插入克隆载体构建重组质粒后基因测序及序列分析。结果克隆出的基因片段编码CaM1全部149个氨基酸,核苷酸序列与多种哺乳动物相应基因的同源性大于90%。获得了该序列的限制性内切酶酶切位点等信息。结论成功获得了豚鼠CaM1基因编码区序列,为进一步重组表达CaM及其功能研究奠定基础。
Aim To clone and sequence the gene of calmodulin 1 （ CaM1 ） in guinea-pig （ Cavia porcellus）; to analyze the bio-information of the cloned gene. Methods Total RNA was extracted from heart tissue of guinea-pig. A DNA fragment of 447 bp from the gene code of CaM1 was amplified by RT-PCR method. Recombinant plasmid was constructed by inserting cDNA into cloning vector before DNA sequencing and sequence analyzing. Results The cloned CaM1 gene encoded 149 amino acid residues. The homology with other mammal＇s relative CaM1 genes was more than 90%. The restriction enzyme positions of this sequence were confirmed. Conehasion The cloning of CaM1 gene in guinea-pig makes it a fundament for CaM expression and function research.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第6期754-757,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30670761,30870907)
