摘要
根据GenBank已发表的拟南芥cbfs转录因子基因序列设计特异性引物,采用PCR和RT-PCR方法,从鸢尾科野生花卉马蔺基因组DNA和cDNA中克隆出cbf转录因子基因片段,将其克隆到pMD18-TVector上。经序列测定及分析表明,两种途径得到的cbf基因序列相同,基因全长642bp,可编码213个氨基酸。同源性分析表明该基因的核苷酸序列和推导的氨基酸序列与其它植物cbf类基因具有同源性,尤其与拟南芥cbf1和黄瓜cbf1基因有很高的同源性,氨基酸同源性高达97%。鉴于同cbf1基因高的同源性,初步确定克隆到马蔺cbf1转录因子基因,命名为Ilcbf1,在GenBank中登录号为DQ131497。
Primers were designed according to the sequence of Arabidopsis thaliana cbfs transcription factor genes from the GenBank. Fragment of cbf transcriptional factor gene was isolated from Iris lactea by PCR and RT-PCR, respectively, and then transferred into pMD18-T Vector. The result derived from the sequence analysis showed that the sequences obtained by two approaches were the same. The full-length cDNA was 642 bp long, and encoded a putative protein of 213 amino acids. Homology analysis showed that the nucleotide and the deduced amino acids sequences were homologous to that of other cbf genes from the different species, especially the strongly homology to the cbfl gene of Arabidopsis thaliana, Cucumis sativus and the homology to 97% above. Based on the high homology to cbfl gene, it is confirmed that cbfl transcription factor gene has been cloned from Iris lactea, designated as Ilcbfl, and the accession number in GenBank is DQ131497.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第6期861-866,共6页
Acta Horticulturae Sinica
基金
黑龙江省科技攻关项目(GB06B112-5)
哈尔滨市人才专项基金项目(2006RFLXN007)
黑龙江省研究生创新科研专项项目(YJSCX2005-200HLJ)
