摘要
利用高效液相色谱法测定博落回注射液中血根碱和白屈菜红碱的含量。采用Agilent1100型高效液相色谱仪,色谱柱为Agilent Ecplise XDB-C18(5μm,4.6mm×250mm),流动相为三乙胺溶液-乙腈(75:25,V/V),流速1.0mL/min;检测波长270nm;柱温35℃。结果表明:血根碱在0.5～100μg/mL范围内线性关系良好(R2=0.9999),平均加样回收率(n=3)为96.1%～97.9%,RSD为0.8%～1.8%;白屈菜红碱在0.5～100μg/mL范围内线性关系良好(R2=1),平均加样回收率(n=3)为96.8%～98.8%,RSD为0.6%～1.0%。本法简单快捷,精密度好,可为制定质控标准提供参考。
This article developed an HPLC method for determination of sanguinarine and chelerythrine in macleaya cordata injection. By Agilent 1100 highly effective liquid phase chromatograph, the Agilent Ecplise XDB- C18 （ 5 μm, 4.6 mm× 250 mm） was used as analytical column with a mobile phase consisting of triethylamine solution - acetronitrile （75 ： 25, V/V）. The flow rate was 1.0 mL/min with uheaviolet detection wavelength at 270 nm and column temperature was 35℃. Finally the founded method linearity of sanguinarin was in the range of 0.5 100 μg/mL, （R2 =0. 999 9）, The average recovery rate （n =3） was 96.1% -97.9%, RSD was 0.8% 1.8% ;And the founded method linearity of chelerythrine was in the range of 0. 5 -100 μg/mL, （R2 = 1 ） , The average recovery rate （n =3） was 96.8% ~98.8%, RSD was 0.6% - 1.0% ; This method was simple,quick, sensitive and can be used as reference for the standard of quality control.
出处
《中国兽药杂志》
2009年第6期15-17,共3页
Chinese Journal of Veterinary Drug
