摘要
目的:探讨转录因子对T细胞分化的影响及它们和细胞因子在系统性红斑狼疮(SLE)发病机制中的作用。方法:利用实时荧光定量PCR技术检测SLE患者、正常人外周血单核细胞(PBMC)中T-bet和GATA-3 mRNA表达状况;用ELISA检测SLE患者、正常人血清中IL-10和TGF-β水平。结果:SLE患者T-bet mRNA表达低于正常人,差异具有显著性(P<0.05);SLE患者GATA-3mRNA表达明显高于正常人,具有统计学差异(P<0.05)。SLE患者IL-10明显高于正常人,差异具有统计学意义(P<0.05);SLE患者TGF-β与正常人差异无统计学意义(P>0.05)。结论:SLE患者Th1/Th2免疫不平衡占优势,调节Th1细胞的转录因子T-bet表达降低,调节Th2细胞的转录因子GATA-3表达升高,细胞因子IL-10水平升高,TGF-β没有明显变化。
Objective： To investigate the effect of transcription factors on the differentiation of T cells, and the role of transcription factors and cytokines in the pathogenesis of SLE. Methods： The expression of transcription factors T-bet and GATA-3 in PBMC from patients with SLE and healthy controls was detected by fluorescent real-time quantitative PCR, the level of IL-10 and TGF-β in serum from patients with SLE and healthy controls was detected by ELISA. Results： Fluoresent real-time quantitative PCR showed T-bet mRNA expression in patients with SLE was lower than that in controls, the differences between patient with SLE and healthy controls were significant. GATA-3 mRNA expression in patients with SLE was higher than that in controls, the differences between patients with SLE and healthy controls were significant. ELISA showed the quantity of IL-10 in serum in patients with SLE was significantly higher than that in healthy controls, and there were no significantly differences of TGF-β between patients and healthy controls. Conclusion： Immunity in patients with SLE loses balance between Th1/Th2, gene expression level of Th1 associated transcription factors （T-bet） are decreased, in contrast, GATA-3 that regulates Th2 cell differentiation are significantly increased. The quantity of IL-10 in patients with SLE is increased and there are no significant differences in the quantity of TGF-β.
出处
《天津医科大学学报》
2009年第2期210-212,共3页
Journal of Tianjin Medical University
