摘要
目的探讨逆转录聚合酶链反应(RT-PCR)在脊柱结核临床诊治中的应用价值,从病原学角度出发探讨脊柱结核手术病灶的切除范围。方法用逆转录聚合酶链反应(RT-PCR)检测25例脊柱结核患者病灶硬化壁、硬化壁外"亚正常骨"以及干酪样坏死组织中的结核分枝杆菌Ag85BmRNA。结果干酪样坏死组织、硬化骨组织、亚正常骨组织Ag85BmRNA检测阳性率分别76%(19/25)、64%(16/25)、28%(7/25),组间差异有统计学意义(P<0.05),平均报告时间2d。结论(1)逆转录聚合酶链反应检测脊柱结核病灶中结核分枝杆菌,具有快速、灵敏、特异性高等特点;(2)脊柱结核病灶硬化壁中存留具有生物活性的结核菌;(3)亚正常骨组织应根据具体情况并结合影像资料判断切除范围。
Objective To investigate application value of the reverse transcription polymerase chain reaction （RT- PCR） in clinical diagnosis and treatment of spinal tuberculosis. And to explore the scope of surgical resection of spinal tuberculosis from the etiological point of view. Methods The RT - PCR was used for detection of mycobacterium tuberculosis Ag85BmRNA in 25 cases with spinal tuberculosis of sclerosis lesions wall, outside wall hardening ＂sub - normal bone,＂ as well as the cheese - like necrotic tissue. Resuits Ag85BmRNA positive detection rates in caseous necrosis tissue, bone tissue sclerosis, sub - normal bone tissue were respectively 76% （19/25）, 64% （16/25） and 28% （7/25）, and there were group significant difference among the groups （P 〈0.05）. Conclusion Reverse transcription polymerase chain reaction detection in the focus of spinal tuberculosis in mycobacterium tuberculosis is rapid, sensitive and specific high; There is biologically active tuberculosis in the focus of spinal tuberculosis in the left wall hardening; Sub - normal bone tissue should be based on the specific circumstances of combined image data to determine the scope of resection.
出处
《宁夏医学杂志》
CAS
2009年第6期492-493,共2页
Ningxia Medical Journal
