摘要
本文采用HRP逆行示踪与谷氨酸(Glu)和γ-氨基丁酸(GABA)免疫细胞化学结合法对Wistar大鼠额叶和枕叶皮质内胼胝体神经元的化学性质进行了研究。结果显示,含Glu的胼胝体神经元为大、中型锥体细胞,主要分布于皮质Ⅱ/Ⅲ、Ⅴ和Ⅵ层,并有成群分布的倾向。同时发现含GABA的胼胝体神经元。它们为中等大小的非锥体细胞,胞体为圓形、椭圆形或梭形,绝大部分位于皮质Ⅴ和Ⅵ层,也有成群分布的倾向。含GABA的胼胝体神经元的百分比(在额叶和枕叶皮质分别约为8％在和10％)比含Glu的胼胝体神经元的百分比(在额叶和枕叶皮质分别约为17％和29％)少得多。本文从形态学上进一步证实相当一部分胼胝体神经元使用Glu作为兴奋性递质;首次证实一些胼胝体神经元含抑制性递质GABA。此外,含GABA的胼胝体神经元的存在提示,至少在大鼠大脑皮质内,一些含GABA神经元有比局部回路神经元较长的投射。结合本文和其他作者的实验结果,我们认为胼胝体系统是一个多递质系统,胼胝体系统中神经递质以及它们之间的相互作用的多样化可能是胼胝体系统具有复杂和高级神经机能的神经解剖学基础之一。
Chemical characteristics of callosal neurons in the frontal and occipital cortex of Wistar rat were studied by means of combined method of HRP retrograde tracing andimmunocytochemistry of glutamate (Glu) and γ-aminobutyric acid (GABA). Glutamate containing callosal neurons were large or medium sized pyramidal cells and mainly localized in layers Ⅱ/Ⅲ, Ⅴ and Ⅵ. They tended to appear in clusters. GABA containing callosal neurons were also found. They were medium sized nonpyramidal cells with round, elliptic, or fusiform soma and were mostly localized in layers Ⅴ and Ⅵ. They also tended to appear in clusters. The percentage of GABA containing callosal neurons (about 8% and 10% in frontal and occipital cortex respectively)was much less than that of glutamate containing callosal neurons (about 17% and 29% in frontal and occipital cortex respectively). These results further confirmed that part of callosal cells used glutamate as an excitatory transmitter and firstly confirmed that some callosal neurons contained an inhibitory transmitter GABA morphologically. In view of the existence of GABA containing callosal neurons, it also suggested that at least in rat cerebral cortex,some GABA containing neurons had longer projections than local circuit neurons. According to the results of ours and other authors, we suggested that callosal system was the one that contained different kinds of neurotransmitters, and that diversification of the transmitters and their interaction in the callosal system was one of the neuroanatomical bases of the complicated and advanced functions of the callosal neurons.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第4期394-398,共5页
Acta Anatomica Sinica
关键词
胼胝体神经元
谷氨酸
氨基丁酸
Callosai neuron
Glutamate
GABA
HRP
Immunocytochemistry
Rat
