摘要
采用荧光染色标记细菌(fluorescence labeled bacteria,FLB)技术,研究山羊瘤胃中原虫对细菌的吞噬速率。试验选用3头装有永久瘘管的徐淮山羊作为瘤胃液供体,采用体外培养法研究在精粗比为1:9的情况下,探讨不同丝兰提取物水平(A组0 mg/L、B组35 mg/L、C组70 mg/L)对瘤胃原虫吞噬细菌速率的影响。荧光镜检与回归分析结果表明:原虫吞噬细菌的速率:A组:339.9 cells/(cell·h);B组:314.7 cells/(cell·h);C组:339.9 cells/(cell·h)。结果表明荧光标记细菌技术能够应用于瘤胃原虫吞噬细菌速率的研究。
Research on the bacterial predation rate by rumen protozoa was carried out using a technique of fluorescence-labeled bacteria in culture in vitro incubation. Three Xuhuai goats fitted with rumen cannula were used to determine the effects of yucca schidigera extract （YSE） at 0 （group A）, 35 （group B） and 70 mg/L （group C） on the determination of bacterial predation rate by rumen protozoa when dietary concentrate to forage ratio was set to 1 ： 9 in the culture. The results showed that the predation rates of rumen protozoa were 339.9 cells/ （ cell· h） in group A, 314.7 cells/（cell · h） in group B, 339.9 cells/（cell, h） in group C, respectively. It was concluded that the fluorescence-labeled bacteria technique would be a potential option for determination of bacterial predation rate by rumen protozoa.
出处
《动物营养学报》
CAS
CSCD
北大核心
2009年第3期417-422,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
National Natural Science Foundation of China(30771567)
关键词
荧光染色标记细菌
吞噬速率
细菌N
Fluorescence-labeled bacteria
Predation rate
Bacterial-N
