摘要
依据猪线粒体DNA中的细胞色素b基因序列设计一对特异性引物,用于检测食物中的猪源成分DNA扩增片段(130bp)可以从猪的基因组中检出而对其它物种(牛、羊、鸡等)样品的检测为阴性,且在各种组织中稳定存在,亦可应用于实时荧光PCR从加工的熟食和混合食物中灵敏地检出痕量猪DNA。
PCR and realtime-PCR methods to detect porcine DNA were developed for identifying hidden pork ingredients in processed foods. The for the species-specific detection primer pair was designed to detect the gene encoding porcine cytochrome b of pork with high sensitivity. The amplified DNA fragment （130 bp） was specifically detected from porcine DNA, while no amplification occurred with other species such as cattle, chicken, sheep, and duck. When the developed PCR and realtime-PCR methods were used for investigating commercial food products, porcine DNA was clearly detected in those containing pork in the list of ingredients. The method is rapid, specific and sensitive, make it applicable for detecting trace amounts of pork in processed foods.
出处
《食品研究与开发》
CAS
北大核心
2009年第6期120-123,共4页
Food Research and Development
