摘要
以裂叶秋海棠(Begonia palmata D.Don)种子获得无菌苗,取其叶片为外植体,对裂叶秋海棠叶片丛生芽诱导和植株再生进行了研究。结果表明:MS+TDZ 0.5 mg/L诱导叶片外植体产生不定芽的诱导效果最佳,诱导率为100%,不定芽个数可达200个/cm2;最佳生根培养基为1/3MS+IBA 0.2 mg/L,2周后生根率达到100%。
Using tissue culture methods, different set of hormone concentration of medium cleft leaf begonia （Begonia palmala D. Don）leaves were studied. Results showed that MS＋6-BA 2. 0 mg/L and MS＋4-PU 0. 5 mg/L induced leaf explants of adventitious buds produced the best results induction, induction rate was 100%, Bud were 150/cm^2, and 120/cm^2 ; one of three strength MS was the best medium for rooting,and rooting rate reached 100% after two weeks.
出处
《北方园艺》
CAS
北大核心
2009年第6期92-94,共3页
Northern Horticulture
基金
广东省自然科学基金资助项目(2007B020704001)
肇庆学院教学研究基金资助项目(0629)
关键词
裂叶秋海棠
组织培养
植株再生
Cleft leaf begonia
Tissue culture
Regeneration
