摘要
[目的]通过愈伤组织诱导途径,建立快速高效的花榈木再生体系。[方法]花榈木成熟种子在MS培养基中萌发获得无菌苗,以幼苗的胚轴为外植体诱导愈伤组织,经继代后进一步诱导不定芽和生根。[结果]诱导花榈木愈伤组织的最适培养基为MS+1.0mg/L2,4-D+0.5mg/LKT,诱导率达96.7%;诱导愈伤组织分化不定芽的培养基为MS+0.5mg/LNAA+0.1mg/LTDZ,其不定芽诱导率为85.0%;平均每块愈伤产生不定芽6.2个;生根的最适培养基为1/2WPM+0.5mg/LNAA+0.5mg/LIBA,生根率为88.9%。炼苗移栽后,成活率可达85.0%。[结论]花榈木胚轴愈伤组织诱导途径的植株再生是一套快速高效的离体再生体系,可为花榈木种质资源保存、转基因、遗传育种等研究提供重要参考。
[ Objective ] A rapid and efficient regeneration system of Ormosia henryi Prain was established by the approach of callus induction. [ Method] Mature seeds of O. henryi sprouted on MS medium, callus was induced with the hypocotyl as explants. After two subcultures, shoots and roots were induced. [ Result ] The best results were achieved on the following media： 96.7% callus induction on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L KT; 85.0% shoot induction on MS medium supplemented with 0.5 mg/L NAA and 0.1 mg/L TDZ, and the mean number of buds per piece of callus was 6.2; 88.9% rooting on 1/2 WPM medium supplemented with 0. 5 mg/L NAA and 0.5 mg/L IBA. The survival rate of the plantlets was 85.0% after being transplanted into soil. [ Conclusion ] Callus Induction and Plant Regeneration from the hypocotyl of O. henry/was a fast and efficient in vitro regeneration system, it will has important theoretical value and realistic significance for reserve of germplasm resource, studies on transgenic engineering and genetics breeding.
出处
《安徽农业科学》
CAS
北大核心
2009年第33期16271-16273,共3页
Journal of Anhui Agricultural Sciences
基金
中国科学院知识创新工程重要方向性项目(KSCX2-YW-N-032)
关键词
愈伤组织
胚轴
花榈木
植株再生
Callus
Hypocotyl
Ormosia henryi Prain
Plant regeneration
