摘要
目的:了解广西壮族及汉族健康人多瘤病毒(BKV)的感染情况,为防治BKV相关性肾病(BKVN)提供理论依据。方法:采集1000例广西壮族和汉族健康人外周血标本,提取外周血淋巴细胞基因组DNA,用巢式PCR方法扩增保守区编码序列,统计分析不同民族、年龄、性别组的BKV-DNA检出率。结果:1000份健康人群PBLs样品中BKV-DNA序列检出率为58.80%,不同民族、年龄、性别组之间的BKV-DNA检出率差异无统计学意义(P>0.05)。结论:BKV在广西壮族及汉族健康人群PBLs中存在较高感染率,分别为58.60%和59.00%,平均为58.80%。进一步证实了,正常人PBLs是BKV在人体内的潜伏靶点及传播工具,应加强对BKV条件致病性的认识,积极防治、减少BKVN的发生率。
Objective：To investigate the prevalence of BK virus in peripheral blood leukocytes （PBLs）of Zhuang and Han healthy individuals in Guangxi so as to describe the transmission of BKV in human body. Methods：DNA in peripheral blood leukocytes from 1000 blood samples of healthy adult individualswas extracted and BKV DNA was examined by nested polymerase chain reaction. The BKV - DNA detection rates were statistically analyzed among the groups of different minorities, ages and sex. Results ： In the 1000 peripheral leukocytes samples, the detection rate of BKV - DNA was 58.80%. There was no significant difference in nationalities, sex or age distribution （ P 〉 0.05 ）. Conclusion ： It shows a high prevalence of BKV in peripheral blood leukocytes of Zhuang （ 58. 60% ） and Han （ 59. 00% ） healthy individuals in Guangxi, with an average of 58, 80%. This study shows that PBLs are the harbors and the transmission carriers for BKV. Awareness of the opportunistic pathogenicity of BKV should be improved so as to decrease and prevent the incidence of BKV nephrosis.
出处
《中国卫生检验杂志》
CAS
2009年第5期1059-1061,共3页
Chinese Journal of Health Laboratory Technology
基金
柳州市科技三项经费资助项目(柳科字2007021317)
